Genetically Engineered Mouse Facility (GEMF)

Equipment/facility: Facility

    Description

    The MD Anderson Genetically Engineered Mouse Facility is fully equipped for the efficient production of transgenic and chimeric mice. The facility is comprised of three rooms dedicated for the housing of mice, one for tissue culture use, one for microinjection and embryo manipulations, a DNA prep lab, a cryopreservation lab and a materials/embryo storage area. The microinjection room is equipped with Leica microinjection scopes and Leica dissecting scopes. There are two biological safety cabinets along with a double stacked tissue culture incubator located separately in a room dedicated for the purposes of tissue culture. There is also a liquid nitrogen storage system for the preservation of ES cells and cryopreservation of mouse embryos and germplasm. All mice are housed under Specific Pathogen Free conditions in three rooms capable of holding over 2,500 mice.
    Eppendorf FemtoJet Injection System
    EBS 4002 Sperm CryoStorage Units
    Pronuclear Injection
    Rederivation
    Blastocyst Injections
    Cryopreservation
    Sperm Cryopreservation
    Charge Through Mouse Resource Facility
    ES Cell Generation
    In Vitro Fertilization
    Electroporation/Gene Targeting
    IVF-Cryopreservation

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    mice
    cryopreservation
    tissue culture
    incubators (equipment)
    germplasm
    spermatozoa
    genetically modified organisms
    cells
    injection
    liquids
    pathogens
    DNA
    nitrogen
    genes