Molecular CytoGenetics Facility (MCF)

Equipment/facility: Facility

    Description

    The T. C. Hsu Molecular Cytogenetics Core facility (MCF) is funded by the CCSG. The primary goal of this facility is to offer conventional and molecular cytogenetic services, including species identification, karyotyping, analysis of genomic instability, fluorescence in situ hybridization and spectral karyotyping. Dr. Guillermina Lozano is the Director and Dr. Asha S. Multani is the Co-Director of the facility.
    Nikon Eclipse 50i microscope and 80i upright microscope
    Applied Spectral Imaging SKY interferometer plus workstation
    Sensys CCD camera for FISH image acquisition and analysis
    PSI Genetiscan cytogenetics workstation with dedicated EM 400 Nikon Microscope, CCD camera and karyotyping software
    Study of genetic instability by chromosome analysis
    Inter-species cell line contamination/Identification by Giemsa Staining
    Detailed Cell line characterization by Karyotyping (banding)
    Giemsa (G), C, Q, R -banding, and silver staining of metaphase chromosomes
    Sister Chromatid Exchange (SCE) analysis
    Meiotic chromosome analysis
    Fluorescence in situ hybridization (FISH) on interphase and metaphase cells using whole chromosome paints, centromere probes, gene mapping with commercial and laboratory-generated DNA probes
    Quantitative FISH (Q-FISH) analysis of telomeric sequences
    Spectral Karyotyping (SKY) in human, mouse and rat cells
    Determine the genetic suitability of mammalian stem cells to be used for cloning purposes

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    Karyotyping
    Cytogenetics
    Metaphase
    Spectral Karyotyping
    Chromosomes
    Cell Line
    Chromosomal Instability
    Silver Staining
    Chromatids
    Paint
    Centromere
    Genomic Instability
    Chromosome Mapping
    Interphase
    Fluorescence In Situ Hybridization
    In Situ Hybridization
    Organism Cloning
    Stem Cells
    DNA