Molecular CytoGenetics Facility (MCF)

The T. C. Hsu Molecular Cytogenetics Core facility (MCF) is funded by the CCSG. The primary goal of this facility is to offer conventional and molecular cytogenetic services, including species identification, karyotyping, analysis of genomic instability, fluorescence in situ hybridization and spectral karyotyping. Dr. Guillermina Lozano is the Director and Dr. Asha S. Multani is the Co-Director of the facility.
Nikon Eclipse 50i microscope and 80i upright microscope
Applied Spectral Imaging SKY interferometer plus workstation
Sensys CCD camera for FISH image acquisition and analysis
PSI Genetiscan cytogenetics workstation with dedicated EM 400 Nikon Microscope, CCD camera and karyotyping software
Study of genetic instability by chromosome analysis
Inter-species cell line contamination/Identification by Giemsa Staining
Detailed Cell line characterization by Karyotyping (banding)
Giemsa (G), C, Q, R -banding, and silver staining of metaphase chromosomes
Sister Chromatid Exchange (SCE) analysis
Meiotic chromosome analysis
Fluorescence in situ hybridization (FISH) on interphase and metaphase cells using whole chromosome paints, centromere probes, gene mapping with commercial and laboratory-generated DNA probes
Quantitative FISH (Q-FISH) analysis of telomeric sequences
Spectral Karyotyping (SKY) in human, mouse and rat cells
Determine the genetic suitability of mammalian stem cells to be used for cloning purposes