Abstract
53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses. We used small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrate that 53BP1 is a key transducer of the DNA damage checkpoint signal. 53BP1 was required for p53 accumulation, G2-M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. 53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brcal and Chk2 but was required for the formation of Brcal foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.
Original language | English (US) |
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Pages (from-to) | 1435-1438 |
Number of pages | 4 |
Journal | Science |
Volume | 298 |
Issue number | 5597 |
DOIs | |
State | Published - Nov 15 2002 |
ASJC Scopus subject areas
- General