53BP1, a mediator of the DNA damage checkpoint

Bin Wang, Shuhei Matsuoka, Phillip B. Carpenter, Stephen J. Elledge

Research output: Contribution to journalArticlepeer-review

490 Scopus citations

Abstract

53BP1 binds to the tumor suppressor protein p53 and has a potential role in DNA damage responses. We used small interfering RNA (siRNA) directed against 53BP1 in mammalian cells to demonstrate that 53BP1 is a key transducer of the DNA damage checkpoint signal. 53BP1 was required for p53 accumulation, G2-M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. 53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins Brcal and Chk2 but was required for the formation of Brcal foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.

Original languageEnglish (US)
Pages (from-to)1435-1438
Number of pages4
JournalScience
Volume298
Issue number5597
DOIs
StatePublished - Nov 15 2002

ASJC Scopus subject areas

  • General

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