A checkpoint-independent mechanism delays entry into mitosis after UV irradiation

Christiane Rothe, Gro Elise Rødland, Silje Anda, Vilte Stonyte, Erik Boye, Sandra Lopez-Aviles, Beáta Grallert

    Research output: Contribution to journalArticle

    Abstract

    When cells are exposed to stress they delay entry into mitosis. The most extensively studied mechanism behind this delay is the DNAdamage- induced G2/M checkpoint. Here, we show the existence of an additional stress-response pathway in Schizosaccharomyces pombe that is independent of the classic ATR/Rad3-dependent checkpoint. This novel mechanism delays entry mitosis independently of the spindle assembly checkpoint and the mitotic kinases Fin1, Ark1 and Plo1. The pathway delays activation of the mitotic cyclin-dependent kinase (CDK) Cdc2 after UV irradiation. Furthermore, we demonstrate that translation of the mitotic cyclin Cdc13 is selectively downregulated after UV irradiation, and we propose that this downregulation of Cdc13 contributes to the delayed activation of Cdc2 and the delayed mitosis.

    Original languageEnglish (US)
    Pages (from-to)4028-4037
    Number of pages10
    JournalJournal of cell science
    Volume130
    Issue number23
    DOIs
    StatePublished - Jan 1 2017

    Keywords

    • Checkpoint
    • Cyclin B
    • G2/M
    • Selective translation
    • UV

    ASJC Scopus subject areas

    • Cell Biology

    Fingerprint Dive into the research topics of 'A checkpoint-independent mechanism delays entry into mitosis after UV irradiation'. Together they form a unique fingerprint.

  • Cite this

    Rothe, C., Rødland, G. E., Anda, S., Stonyte, V., Boye, E., Lopez-Aviles, S., & Grallert, B. (2017). A checkpoint-independent mechanism delays entry into mitosis after UV irradiation. Journal of cell science, 130(23), 4028-4037. https://doi.org/10.1242/jcs.204693