A comprehensive guide for the recognition and classification of distinct stages of hair follicle morphogenesis

Ralf Paus, Sven Müller-Röver, Carina Van Der Veen, Marcus Maurer, Stefan Eichmüller, Gao Ling, Udo Hofmann, Kerstin Foitzik, Lars Mecklenburg, Bori Handjiski

    Research output: Contribution to journalReview article

    398 Scopus citations

    Abstract

    Numerous spontaneous and experimentally induced mouse mutations develop a hair phenotype, which is often associated with more or less discrete abnormalities in hair follicle development. In order to recognize these, it is critically important to be able to determine and to classify accurately the major stages of normal murine hair follicle morphogenesis. As an aid, we propose a pragmatic and comprehensive guide, modified after previous suggestions by Hardy, and provide a list of easily recognizable classification criteria, illustrated by representative micrographs. Basic and more advanced criteria are distinguished, the former being applicable to all mouse strains and requiring only simple histologic stains (hematoxylin and eosin, Giemsa, periodic acid Schiff, alkaline phosphatase activity), the latter serving as auxiliary criteria, which require a pigmented mouse strain (like C57BL/6J) or immunohistochemistry (interleukin-1 receptor type I, transforming growth factor-β receptor type II). In addition, we present simplified, computer-generated schematic drawings for the standardized recording and reporting of gene and antigen expression patterns during hair follicle development. This classification aid serves as a basic introduction into the field of hair follicle morphogenesis, aims at standardizing the presentation of related hair research data, and should become a useful tool when screening new mouse mutants for discrete abnormalities of hair follicle morphogenesis (compared with the respective wild type) in a highly reproducible, easily applicable, and quantifiable manner.

    Original languageEnglish (US)
    Pages (from-to)523-532
    Number of pages10
    JournalJournal of Investigative Dermatology
    Volume113
    Issue number4
    DOIs
    StatePublished - Jan 1 1999

    Keywords

    • 6J mouse
    • Alkaline phosphatase
    • C57BL
    • Dermal papilla
    • Interleukin-1 receptor
    • Transforming growth factor-β receptor

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Dermatology
    • Cell Biology

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    Paus, R., Müller-Röver, S., Van Der Veen, C., Maurer, M., Eichmüller, S., Ling, G., Hofmann, U., Foitzik, K., Mecklenburg, L., & Handjiski, B. (1999). A comprehensive guide for the recognition and classification of distinct stages of hair follicle morphogenesis. Journal of Investigative Dermatology, 113(4), 523-532. https://doi.org/10.1046/j.1523-1747.1999.00740.x