A convenient cloning vector containing the GAL4 DNA-binding domain

Loretta Raycroft, Guillermina Lozano

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A DNA fragment encoding the yeast GAL4 DNA-binding domain (amino acids 3-147) was cloned into a convenient vector. This vector contains unique restriction sites at both the 5' and 3' ends and allows the generation effusion proteins containing the GAL4 DNA-binding domain. These fusion proteins can be tested for their ability to activate transcription.

Original languageEnglish (US)
Pages (from-to)143-144
Number of pages2
JournalGene
Volume118
Issue number1
DOIs
StatePublished - Sep 1 1992

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Keywords

  • Recombinant DNA
  • fusion proteins
  • trans-activation

ASJC Scopus subject areas

  • Genetics

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