A convenient cloning vector containing the GAL4 DNA-binding domain

Loretta Raycroft; Guillermina Lozano

doi:10.1016/0378-1119(92)90262-N

A convenient cloning vector containing the GAL4 DNA-binding domain

Loretta Raycroft, Guillermina Lozano

Genetics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A DNA fragment encoding the yeast GAL4 DNA-binding domain (amino acids 3-147) was cloned into a convenient vector. This vector contains unique restriction sites at both the 5' and 3' ends and allows the generation effusion proteins containing the GAL4 DNA-binding domain. These fusion proteins can be tested for their ability to activate transcription.

Original language	English (US)
Pages (from-to)	143-144
Number of pages	2
Journal	Gene
Volume	118
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(92)90262-N
State	Published - Sep 1 1992

Keywords

Recombinant DNA
fusion proteins
trans-activation

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(92)90262-N

Cite this

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title = "A convenient cloning vector containing the GAL4 DNA-binding domain",

abstract = "A DNA fragment encoding the yeast GAL4 DNA-binding domain (amino acids 3-147) was cloned into a convenient vector. This vector contains unique restriction sites at both the 5' and 3' ends and allows the generation effusion proteins containing the GAL4 DNA-binding domain. These fusion proteins can be tested for their ability to activate transcription.",

keywords = "Recombinant DNA, fusion proteins, trans-activation",

author = "Loretta Raycroft and Guillermina Lozano",

year = "1992",

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doi = "10.1016/0378-1119(92)90262-N",

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AU - Raycroft, Loretta

AU - Lozano, Guillermina

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N2 - A DNA fragment encoding the yeast GAL4 DNA-binding domain (amino acids 3-147) was cloned into a convenient vector. This vector contains unique restriction sites at both the 5' and 3' ends and allows the generation effusion proteins containing the GAL4 DNA-binding domain. These fusion proteins can be tested for their ability to activate transcription.

AB - A DNA fragment encoding the yeast GAL4 DNA-binding domain (amino acids 3-147) was cloned into a convenient vector. This vector contains unique restriction sites at both the 5' and 3' ends and allows the generation effusion proteins containing the GAL4 DNA-binding domain. These fusion proteins can be tested for their ability to activate transcription.

KW - Recombinant DNA

KW - fusion proteins

KW - trans-activation

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DO - 10.1016/0378-1119(92)90262-N

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JF - Gene

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ER -

A convenient cloning vector containing the GAL4 DNA-binding domain

Abstract

Keywords

ASJC Scopus subject areas

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