A general route for post-translational cyclization of mRNA display libraries

Steven W. Millward; Terry T. Takahashi; Richard W. Roberts

doi:10.1021/ja054373h

A general route for post-translational cyclization of mRNA display libraries

Steven W. Millward, Terry T. Takahashi, Richard W. Roberts

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

Cyclic peptides are attractive scaffolds for the design of conformationally constrained molecular therapeutics. Previously, biological display libraries could only be cyclized via disulfide bonds, which are labile and can be reduced in an intracellular environment. In this paper, we construct high diversity, covalently cyclized mRNA display libraries (> 10¹³ sequences) and analyze the cyclization reaction using MALDI-TOF MS and unnatural amino acid incorporation. Our route allows the extent of cyclization to be evaluated quantitatively and is broadly applicable to a variety of cyclization chemistries.

Original language	English (US)
Pages (from-to)	14142-14143
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	127
Issue number	41
DOIs	https://doi.org/10.1021/ja054373h
State	Published - Oct 19 2005
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja054373h

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A general route for post-translational cyclization of mRNA display libraries

Abstract

ASJC Scopus subject areas

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