A human papillomavirus testing system in women with abnormal pap results: A comparison study with follow-up biopsies

Ming Guo; Shobha J. Patel; Marilyn Chovanec; Jee Jan Yee; Emily Tarco; Therese B. Bevers; Keith Anderson; Nour Sneige

doi:10.1159/000325838

A human papillomavirus testing system in women with abnormal pap results: A comparison study with follow-up biopsies

Ming Guo, Shobha J. Patel, Marilyn Chovanec, Jee Jan Yee, Emily Tarco, Therese B. Bevers, Keith Anderson, Nour Sneige

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Objective: To evaluate the efficacy of INFORM HPV using the SurePath collection method in women whose Pap tests indicated abnormal results. Study Design: Ninety-two women from the gynecology clinics at The University of Texas M. D. Anderson Cancer Center who had Pap tests and underwent follow-up biopsies were selected for the study. This included 51 women with atypical squamous cells of undetermined significance (ASCUS), 23 women with low-grade squamous intraepithelial lesion (LSIL), 15 women with high-grade squamous intraepithelial lesion (HSIL) and 3 women with negative Pap results. The INFORM HPV, an in situ hybridization assay, testing for oncogenic types of HPV was performed, and the results were compared with follow-up biopsies. Results: The positive rate of the INFORM HPV increased with higher grades of cytology diagnoses. The sensitivity of the INFORM HPV testing for predicting high-grade cervical intraepithelial neoplasia (CIN 2/3) also increased with high-er grades of cytology diagnoses. A negative predictive value (NPV) of 94.9% and a specificity of 80.4% for predicting CIN 2/3 were observed in the ASCUS group. Conclusion: Using SurePath Pap specimens, the INFORM HPV lacks sufficient sensitivity and NPV for predicting CIN 2/3 in women with ASCUS. Therefore, use of the test as a triage tool is limited.

Original language	English (US)
Pages (from-to)	749-754
Number of pages	6
Journal	Acta Cytologica
Volume	51
Issue number	5
DOIs	https://doi.org/10.1159/000325838
State	Published - 2007

Keywords

Cervical intraepithelial neoplasm
Human papillomavirus
INFORM HPV
In situ hybridization

ASJC Scopus subject areas

Pathology and Forensic Medicine
Histology

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10.1159/000325838

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title = "A human papillomavirus testing system in women with abnormal pap results: A comparison study with follow-up biopsies",

abstract = "Objective: To evaluate the efficacy of INFORM HPV using the SurePath collection method in women whose Pap tests indicated abnormal results. Study Design: Ninety-two women from the gynecology clinics at The University of Texas M. D. Anderson Cancer Center who had Pap tests and underwent follow-up biopsies were selected for the study. This included 51 women with atypical squamous cells of undetermined significance (ASCUS), 23 women with low-grade squamous intraepithelial lesion (LSIL), 15 women with high-grade squamous intraepithelial lesion (HSIL) and 3 women with negative Pap results. The INFORM HPV, an in situ hybridization assay, testing for oncogenic types of HPV was performed, and the results were compared with follow-up biopsies. Results: The positive rate of the INFORM HPV increased with higher grades of cytology diagnoses. The sensitivity of the INFORM HPV testing for predicting high-grade cervical intraepithelial neoplasia (CIN 2/3) also increased with high-er grades of cytology diagnoses. A negative predictive value (NPV) of 94.9% and a specificity of 80.4% for predicting CIN 2/3 were observed in the ASCUS group. Conclusion: Using SurePath Pap specimens, the INFORM HPV lacks sufficient sensitivity and NPV for predicting CIN 2/3 in women with ASCUS. Therefore, use of the test as a triage tool is limited.",

keywords = "Cervical intraepithelial neoplasm, Human papillomavirus, INFORM HPV, In situ hybridization",

author = "Ming Guo and Patel, {Shobha J.} and Marilyn Chovanec and Yee, {Jee Jan} and Emily Tarco and Bevers, {Therese B.} and Keith Anderson and Nour Sneige",

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T2 - A comparison study with follow-up biopsies

AU - Guo, Ming

AU - Patel, Shobha J.

AU - Chovanec, Marilyn

AU - Yee, Jee Jan

AU - Tarco, Emily

AU - Bevers, Therese B.

AU - Anderson, Keith

AU - Sneige, Nour

PY - 2007

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N2 - Objective: To evaluate the efficacy of INFORM HPV using the SurePath collection method in women whose Pap tests indicated abnormal results. Study Design: Ninety-two women from the gynecology clinics at The University of Texas M. D. Anderson Cancer Center who had Pap tests and underwent follow-up biopsies were selected for the study. This included 51 women with atypical squamous cells of undetermined significance (ASCUS), 23 women with low-grade squamous intraepithelial lesion (LSIL), 15 women with high-grade squamous intraepithelial lesion (HSIL) and 3 women with negative Pap results. The INFORM HPV, an in situ hybridization assay, testing for oncogenic types of HPV was performed, and the results were compared with follow-up biopsies. Results: The positive rate of the INFORM HPV increased with higher grades of cytology diagnoses. The sensitivity of the INFORM HPV testing for predicting high-grade cervical intraepithelial neoplasia (CIN 2/3) also increased with high-er grades of cytology diagnoses. A negative predictive value (NPV) of 94.9% and a specificity of 80.4% for predicting CIN 2/3 were observed in the ASCUS group. Conclusion: Using SurePath Pap specimens, the INFORM HPV lacks sufficient sensitivity and NPV for predicting CIN 2/3 in women with ASCUS. Therefore, use of the test as a triage tool is limited.

AB - Objective: To evaluate the efficacy of INFORM HPV using the SurePath collection method in women whose Pap tests indicated abnormal results. Study Design: Ninety-two women from the gynecology clinics at The University of Texas M. D. Anderson Cancer Center who had Pap tests and underwent follow-up biopsies were selected for the study. This included 51 women with atypical squamous cells of undetermined significance (ASCUS), 23 women with low-grade squamous intraepithelial lesion (LSIL), 15 women with high-grade squamous intraepithelial lesion (HSIL) and 3 women with negative Pap results. The INFORM HPV, an in situ hybridization assay, testing for oncogenic types of HPV was performed, and the results were compared with follow-up biopsies. Results: The positive rate of the INFORM HPV increased with higher grades of cytology diagnoses. The sensitivity of the INFORM HPV testing for predicting high-grade cervical intraepithelial neoplasia (CIN 2/3) also increased with high-er grades of cytology diagnoses. A negative predictive value (NPV) of 94.9% and a specificity of 80.4% for predicting CIN 2/3 were observed in the ASCUS group. Conclusion: Using SurePath Pap specimens, the INFORM HPV lacks sufficient sensitivity and NPV for predicting CIN 2/3 in women with ASCUS. Therefore, use of the test as a triage tool is limited.

KW - Cervical intraepithelial neoplasm

KW - Human papillomavirus

KW - INFORM HPV

KW - In situ hybridization

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A human papillomavirus testing system in women with abnormal pap results: A comparison study with follow-up biopsies

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