TY - GEN
T1 - A micro- flow cytometer based on dielectrophoretic particle focusing
AU - Yu, Choongho
AU - Shi, Li
AU - Vykoukal, Jody
AU - Gascoyne, Peter R.C.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2003
Y1 - 2003
N2 - This paper reports an on-chip micro- flow cytometer that focuses cells to the center region of a microchannel by a negative dielectrophoretic force generated by ac fringing fields from microelectrodes. This design eliminates the sheath flow that makes the conventional cytometer sophisticated and difficult to operate. The micro- cytometer has a circular microfluidic channel that is fabricated by isotropic etching of soda lime glass wafers and a subsequent wafer bonding process. Electrode arrays are patterned inside the circular channel to facilitate dielectrophoretic particle focusing. We have demonstrated that the device can focus micro-beads into the center of the channel, and are integrating optical components with the micro- cytometer to realize a handheld device with integrated fluorescence detection, and will test the micro-cytometer for blood cell profiling and tumor cell detection.
AB - This paper reports an on-chip micro- flow cytometer that focuses cells to the center region of a microchannel by a negative dielectrophoretic force generated by ac fringing fields from microelectrodes. This design eliminates the sheath flow that makes the conventional cytometer sophisticated and difficult to operate. The micro- cytometer has a circular microfluidic channel that is fabricated by isotropic etching of soda lime glass wafers and a subsequent wafer bonding process. Electrode arrays are patterned inside the circular channel to facilitate dielectrophoretic particle focusing. We have demonstrated that the device can focus micro-beads into the center of the channel, and are integrating optical components with the micro- cytometer to realize a handheld device with integrated fluorescence detection, and will test the micro-cytometer for blood cell profiling and tumor cell detection.
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U2 - 10.1115/imece2003-42795
DO - 10.1115/imece2003-42795
M3 - Conference contribution
AN - SCOPUS:1942423750
SN - 0791837211
SN - 9780791837214
T3 - American Society of Mechanical Engineers, Micro-Electromechanical Systems Division Publication (MEMS)
SP - 545
EP - 549
BT - Micro-Electro-Mechanical Systems (MEMS) - 2003
PB - American Society of Mechanical Engineers
T2 - 2003 ASME International Mechanical Engineering Congress
Y2 - 15 November 2003 through 21 November 2003
ER -