A micro- flow cytometer based on dielectrophoretic particle focusing

Choongho Yu, Li Shi, Jody Vykoukal, Peter R.C. Gascoyne

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports an on-chip micro- flow cytometer that focuses cells to the center region of a microchannel by a negative dielectrophoretic force generated by ac fringing fields from microelectrodes. This design eliminates the sheath flow that makes the conventional cytometer sophisticated and difficult to operate. The micro- cytometer has a circular microfluidic channel that is fabricated by isotropic etching of soda lime glass wafers and a subsequent wafer bonding process. Electrode arrays are patterned inside the circular channel to facilitate dielectrophoretic particle focusing. We have demonstrated that the device can focus micro-beads into the center of the channel, and are integrating optical components with the micro- cytometer to realize a handheld device with integrated fluorescence detection, and will test the micro-cytometer for blood cell profiling and tumor cell detection.

Original languageEnglish (US)
Title of host publicationMicro-Electro-Mechanical Systems (MEMS) - 2003
PublisherAmerican Society of Mechanical Engineers
Pages545-549
Number of pages5
ISBN (Print)0791837211, 9780791837214
DOIs
StatePublished - 2003
Event2003 ASME International Mechanical Engineering Congress - Washington, DC, United States
Duration: Nov 15 2003Nov 21 2003

Publication series

NameAmerican Society of Mechanical Engineers, Micro-Electromechanical Systems Division Publication (MEMS)
Volume5

Other

Other2003 ASME International Mechanical Engineering Congress
Country/TerritoryUnited States
CityWashington, DC
Period11/15/0311/21/03

ASJC Scopus subject areas

  • General Engineering

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