A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture

Shingo Fujioka, Sampetrean Oltea, Hideyuki Saya, Ryo Sudo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper presents a microfluidic three-dimensional (3D) platform to investigate behaviors of glioma-initiating cells (GIC) cocultured with human umbilical vein endothelial cells (HUVEC). In this platform, these cells were cultured in microenvironments similar to in vivo. Especially, our experiments have been performed with GIC established by gene transfection [1], which is different from conventional tumor cell lines. Our results demonstrate that biochemical factors secreted by HUVEC attracts glioma invasion.

Original languageEnglish (US)
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1075-1077
Number of pages3
ISBN (Print)9781632666246
StatePublished - Jan 1 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Endothelial cells
Microfluidics
Assays
Cells
Tumors
Genes
Experiments

Keywords

  • Glioma-initiating cells
  • Invasion
  • Microfluidic device

ASJC Scopus subject areas

  • Bioengineering

Cite this

Fujioka, S., Oltea, S., Saya, H., & Sudo, R. (2013). A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1075-1077). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.

A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. / Fujioka, Shingo; Oltea, Sampetrean; Saya, Hideyuki; Sudo, Ryo.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 1075-1077 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fujioka, S, Oltea, S, Saya, H & Sudo, R 2013, A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 2, Chemical and Biological Microsystems Society, pp. 1075-1077, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Fujioka S, Oltea S, Saya H, Sudo R. A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 1075-1077. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Fujioka, Shingo ; Oltea, Sampetrean ; Saya, Hideyuki ; Sudo, Ryo. / A microfluidic invasion assay for glioma-initiating cells in three-dimensional culture. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 1075-1077 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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