A model for the topology of active ribosomal RNA genes

Serguei Denissov, Frédéric Lessard, Christine Mayer, Victor Stefanovsky, Marc Van Driel, Ingrid Grummt, Tom Moss, Hendrik G. Stunnenberg

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The Christmas tree view of active ribosomal RNA (rRNA) genes suggests a gene topology in which a large number of nascent rRNA transcripts are prevented from intertwining. The way in which this is achieved has remained unclear. By using a combination of chromatin immunoprecipitation and chromosome conformation capture techniques, we show that the promoter, upstream region and terminator R3 of active rRNA genes are held together spatially throughout the cell cycle, forming a stable core around which the transcribed region is organized. We suggest a new core-helix model for the topology of rRNA genes, that provides a structural basis for the productive synthesis or rRNA.

Original languageEnglish (US)
Pages (from-to)231-237
Number of pages7
JournalEMBO reports
Volume12
Issue number3
DOIs
StatePublished - Mar 1 2011

Fingerprint

Ribosomal RNA
rRNA Genes
Genes
Topology
Chromatin Immunoprecipitation
Genetic Promoter Regions
Cell Cycle
Genetic Terminator Regions
Chromosomes
Chromatin
Conformations
Cells

Keywords

  • Christmas trees
  • RNA polymerase I
  • core-helix model
  • rDNA topology
  • rRNA genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Denissov, S., Lessard, F., Mayer, C., Stefanovsky, V., Van Driel, M., Grummt, I., ... Stunnenberg, H. G. (2011). A model for the topology of active ribosomal RNA genes. EMBO reports, 12(3), 231-237. https://doi.org/10.1038/embor.2011.8

A model for the topology of active ribosomal RNA genes. / Denissov, Serguei; Lessard, Frédéric; Mayer, Christine; Stefanovsky, Victor; Van Driel, Marc; Grummt, Ingrid; Moss, Tom; Stunnenberg, Hendrik G.

In: EMBO reports, Vol. 12, No. 3, 01.03.2011, p. 231-237.

Research output: Contribution to journalArticle

Denissov, S, Lessard, F, Mayer, C, Stefanovsky, V, Van Driel, M, Grummt, I, Moss, T & Stunnenberg, HG 2011, 'A model for the topology of active ribosomal RNA genes', EMBO reports, vol. 12, no. 3, pp. 231-237. https://doi.org/10.1038/embor.2011.8
Denissov S, Lessard F, Mayer C, Stefanovsky V, Van Driel M, Grummt I et al. A model for the topology of active ribosomal RNA genes. EMBO reports. 2011 Mar 1;12(3):231-237. https://doi.org/10.1038/embor.2011.8
Denissov, Serguei ; Lessard, Frédéric ; Mayer, Christine ; Stefanovsky, Victor ; Van Driel, Marc ; Grummt, Ingrid ; Moss, Tom ; Stunnenberg, Hendrik G. / A model for the topology of active ribosomal RNA genes. In: EMBO reports. 2011 ; Vol. 12, No. 3. pp. 231-237.
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