Abstract
Selective intra-bronchial instillation of hydrochloric acid (HCl) to the murine left mainstem bronchus causes acute tissue injury with histopathologic findings similar to human acute respiratory distress syndrome (ARDS). The resulting alveolar edema, alveolar-capillary barrier damage, and leukocyte infiltration predominantly affect the left lung, preserving the right lung as an uninjured control and allowing animals to survive. This model of self-limited acute lung injury enables investigation of tissue resolution mechanisms, such as macrophage efferocytosis of apoptotic neutrophils and restitution of alveolar-capillary barrier integrity. This model has helped identify important roles for resolution agonists, including specialized pro-resolving mediators (SPMs), providing a foundation for the development of new therapeutic approaches for patients with ARDS.
Original language | English (US) |
---|---|
Article number | e60024 |
Journal | Journal of Visualized Experiments |
Volume | 2019 |
Issue number | 150 |
DOIs | |
State | Published - Aug 2019 |
Keywords
- Acid aspiration
- Acute lung injury
- Acute respiratory distress syndrome (ARDS)
- Immunology and Infection
- Issue 150
- Resolution
- Selective intra-bronchial instillation
- Unilateral
ASJC Scopus subject areas
- General Neuroscience
- General Chemical Engineering
- General Biochemistry, Genetics and Molecular Biology
- General Immunology and Microbiology