A monoclonal antibody to Shigella dysenteriae serotype 13 cross-reacting with Shiga toxin

Firduasi Qadri; Tasnim Azim; Anwar Hossain; Dilara Islam; Gabriel Mondol; Shah Mohammad Faruque; Manuel John Albert

doi:10.1111/j.1574-6968.1993.tb06055.x

A monoclonal antibody to Shigella dysenteriae serotype 13 cross-reacting with Shiga toxin

Firduasi Qadri
, Tasnim Azim
, Anwar Hossain
, Dilara Islam
, Gabriel Mondol
, Shah Mohammad Faruque
, Manuel John Albert

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A monoclonal antibody (mAb ICT6) was produced against the newly described Shigella dysenteriae serotype type 13. The mAb was of IgM isotype and recognized purified Shiga toxin in ELISA and immunoblot. It also recognized periplasmic extract S. dysenteriae type 13 in immunoblot as did an affinity-purified polyclonal rabbit antiserum and a previously described monoclonal antibody to the B subunit of Shiga toxin. The mAb ICT6 did not neutralize the cytotoxic effects or S. dysenteriae type 13, Shiga toxin or periplasmic extracts of S. dysenteriae type 1 for HeLa cells.

Original language	English (US)
Pages (from-to)	343-347
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	107
Issue number	2-3
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06055.x
State	Published - Mar 1 1993
Externally published	Yes

Keywords

Antibody
Cross-reaction
Shiga toxin
Shigella dysenteriae 13

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1993.tb06055.x

Cite this

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title = "A monoclonal antibody to Shigella dysenteriae serotype 13 cross-reacting with Shiga toxin",

abstract = "A monoclonal antibody (mAb ICT6) was produced against the newly described Shigella dysenteriae serotype type 13. The mAb was of IgM isotype and recognized purified Shiga toxin in ELISA and immunoblot. It also recognized periplasmic extract S. dysenteriae type 13 in immunoblot as did an affinity-purified polyclonal rabbit antiserum and a previously described monoclonal antibody to the B subunit of Shiga toxin. The mAb ICT6 did not neutralize the cytotoxic effects or S. dysenteriae type 13, Shiga toxin or periplasmic extracts of S. dysenteriae type 1 for HeLa cells.",

keywords = "Antibody, Cross-reaction, Shiga toxin, Shigella dysenteriae 13",

author = "Firduasi Qadri and Tasnim Azim and Anwar Hossain and Dilara Islam and Gabriel Mondol and Faruque, \{Shah Mohammad\} and Albert, \{Manuel John\}",

year = "1993",

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doi = "10.1111/j.1574-6968.1993.tb06055.x",

language = "English (US)",

volume = "107",

pages = "343--347",

journal = "FEMS Microbiology Letters",

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T1 - A monoclonal antibody to Shigella dysenteriae serotype 13 cross-reacting with Shiga toxin

AU - Qadri, Firduasi

AU - Azim, Tasnim

AU - Hossain, Anwar

AU - Islam, Dilara

AU - Mondol, Gabriel

AU - Faruque, Shah Mohammad

AU - Albert, Manuel John

PY - 1993/3/1

Y1 - 1993/3/1

N2 - A monoclonal antibody (mAb ICT6) was produced against the newly described Shigella dysenteriae serotype type 13. The mAb was of IgM isotype and recognized purified Shiga toxin in ELISA and immunoblot. It also recognized periplasmic extract S. dysenteriae type 13 in immunoblot as did an affinity-purified polyclonal rabbit antiserum and a previously described monoclonal antibody to the B subunit of Shiga toxin. The mAb ICT6 did not neutralize the cytotoxic effects or S. dysenteriae type 13, Shiga toxin or periplasmic extracts of S. dysenteriae type 1 for HeLa cells.

AB - A monoclonal antibody (mAb ICT6) was produced against the newly described Shigella dysenteriae serotype type 13. The mAb was of IgM isotype and recognized purified Shiga toxin in ELISA and immunoblot. It also recognized periplasmic extract S. dysenteriae type 13 in immunoblot as did an affinity-purified polyclonal rabbit antiserum and a previously described monoclonal antibody to the B subunit of Shiga toxin. The mAb ICT6 did not neutralize the cytotoxic effects or S. dysenteriae type 13, Shiga toxin or periplasmic extracts of S. dysenteriae type 1 for HeLa cells.

KW - Antibody

KW - Cross-reaction

KW - Shiga toxin

KW - Shigella dysenteriae 13

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UR - https://www.scopus.com/pages/publications/0027466278#tab=citedBy

U2 - 10.1111/j.1574-6968.1993.tb06055.x

DO - 10.1111/j.1574-6968.1993.tb06055.x

M3 - Article

C2 - 8472916

AN - SCOPUS:0027466278

SN - 0378-1097

VL - 107

SP - 343

EP - 347

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 2-3

ER -

A monoclonal antibody to Shigella dysenteriae serotype 13 cross-reacting with Shiga toxin

Abstract

Keywords

ASJC Scopus subject areas

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