A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging

Go Shioi; Hiroshi Kiyonari; Takaya Abe; Kazuki Nakao; Toshihiko Fujimori; Chuan Wei Jang; Cheng Chiu Huang; Haruhiko Akiyama; Richard R. Behringer; Shinichi Aizawa

doi:10.1002/dvg.20758

A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging

Go Shioi
, Hiroshi Kiyonari
, Takaya Abe
, Kazuki Nakao
, Toshihiko Fujimori
, Chuan Wei Jang
, Cheng Chiu Huang
, Haruhiko Akiyama
, Richard R. Behringer
, Shinichi Aizawa

Genetics

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.

Original language	English (US)
Pages (from-to)	570-578
Number of pages	9
Journal	Genesis (United States)
Volume	49
Issue number	7
DOIs	https://doi.org/10.1002/dvg.20758
State	Published - Jul 2011

Keywords

Conditional cell labeling
Dual cell labeling
Membrane labeling
Nuclear labeling
Rosa26 locus

ASJC Scopus subject areas

Genetics
Endocrinology
Cell Biology

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10.1002/dvg.20758

Cite this

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title = "A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging",

abstract = "Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.",

keywords = "Conditional cell labeling, Dual cell labeling, Membrane labeling, Nuclear labeling, Rosa26 locus",

author = "Go Shioi and Hiroshi Kiyonari and Takaya Abe and Kazuki Nakao and Toshihiko Fujimori and Jang, \{Chuan Wei\} and Huang, \{Cheng Chiu\} and Haruhiko Akiyama and Behringer, \{Richard R.\} and Shinichi Aizawa",

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T1 - A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging

AU - Shioi, Go

AU - Kiyonari, Hiroshi

AU - Abe, Takaya

AU - Nakao, Kazuki

AU - Fujimori, Toshihiko

AU - Jang, Chuan Wei

AU - Huang, Cheng Chiu

AU - Akiyama, Haruhiko

AU - Behringer, Richard R.

AU - Aizawa, Shinichi

PY - 2011/7

Y1 - 2011/7

N2 - Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.

AB - Live-imaging is an essential tool to visualize live cells and monitor their behaviors during development. This technology demands a variety of mouse reporter lines, each uniquely expressing a fluorescent protein. Here, we developed an R26R-RG reporter mouse line that conditionally and simultaneously expresses mCherry and EGFP in nuclei and plasma membranes, respectively, from the Rosa26 locus. The intensity and resolution of mCherry nuclear localization and EGFP membrane localization were demonstrated to be sufficient for live-imaging with embryos that express RG (mCherry and EGFP) ubiquitously and specifically in fetal Sertoli cells. The conditional R26R-RG reporter mouse line should be a useful tool for labeling nuclei and membranes simultaneously in distinct cell populations.

KW - Conditional cell labeling

KW - Dual cell labeling

KW - Membrane labeling

KW - Nuclear labeling

KW - Rosa26 locus

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UR - https://www.scopus.com/pages/publications/79960135404#tab=citedBy

U2 - 10.1002/dvg.20758

DO - 10.1002/dvg.20758

M3 - Article

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AN - SCOPUS:79960135404

SN - 1526-954X

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JO - Genesis (United States)

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A mouse reporter line to conditionally mark nuclei and cell membranes for in vivo live-imaging

Abstract

Keywords

ASJC Scopus subject areas

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