A Novel Role of Nectins in Inhibition of the E-Cadherin-induced Activation of Rac and Formation of Cell-Cell Adherens Junctions

Takashi Hoshino, Kazuya Shimizu, Tomoyuki Honda, Tomomi Kawakatsu, Taihei Fukuyama, Takeshi Nakamura, Michiyuki Matsuda, Yoshimi Takai

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Nectins are Ca2+-independent immunoglobulin (Ig)-like cell-cell adhesion molecules. The trans-interactions of nectins recruit cadherins to the nectin-based cell-cell adhesion, resulting in formation of cell-cell adherens junctions (AJs) in epithelial cells and fibroblasts. The trans-interaction of E-cadherin induces activation of Rac small G protein, whereas the trans-interactions of nectins induce activation of not only Rac but also Cdc42 small G protein. We showed by the fluorescent resonance energy transfer (FRET) imaging that the trans-interaction of E-cadherin induced dynamic activation and inactivation of Rac, which led to dynamic formation and retraction of lamellipodia. Moreover, we found here that the nectins, which did not trans-interact with other nectins (non-trans-interacting nectins), inhibited the E-cadherin-induced activation of Rac and reduced the velocity of the formation of the E-cadherin-based cell-cell AJs. The inhibitory effect of non-trans-interacting nectins was suppressed by the activation of Cdc42 induced by the trans-interactions of nectins. These results indicate a novel role of nectins in regulation of the E-cadherin-induced activation of Rac and formation of cell-cell AJs.

Original languageEnglish (US)
Pages (from-to)1077-1088
Number of pages12
JournalMolecular Biology of the Cell
Volume15
Issue number3
DOIs
StatePublished - Mar 1 2004

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Adherens Junctions
Intercellular Junctions
Cadherins
Monomeric GTP-Binding Proteins
rac GTP-Binding Proteins
nectins
Pseudopodia
Energy Transfer
Cell Adhesion Molecules
Cell Adhesion
Immunoglobulins
Fibroblasts
Epithelial Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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A Novel Role of Nectins in Inhibition of the E-Cadherin-induced Activation of Rac and Formation of Cell-Cell Adherens Junctions. / Hoshino, Takashi; Shimizu, Kazuya; Honda, Tomoyuki; Kawakatsu, Tomomi; Fukuyama, Taihei; Nakamura, Takeshi; Matsuda, Michiyuki; Takai, Yoshimi.

In: Molecular Biology of the Cell, Vol. 15, No. 3, 01.03.2004, p. 1077-1088.

Research output: Contribution to journalArticle

Hoshino, Takashi ; Shimizu, Kazuya ; Honda, Tomoyuki ; Kawakatsu, Tomomi ; Fukuyama, Taihei ; Nakamura, Takeshi ; Matsuda, Michiyuki ; Takai, Yoshimi. / A Novel Role of Nectins in Inhibition of the E-Cadherin-induced Activation of Rac and Formation of Cell-Cell Adherens Junctions. In: Molecular Biology of the Cell. 2004 ; Vol. 15, No. 3. pp. 1077-1088.
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