A rat cell line derived from DMBA-induced mammary carcinoma

Louis W.C. Chow, Mary N.B. Cheung, Wings T.Y. Loo, X. Y. Guan

    Research output: Contribution to journalArticle

    10 Scopus citations

    Abstract

    A new cell line, designated UHKBR-01, was successfully established from a 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat mammary tumour. DMBA was administered orally at a dose of 4 mg/ml per rat on the first day of the experiment and thereafter at weekly intervals of same dosage, until the rats have reached a weight of around 150-200 g. The tumours grew rapidly after the injection, and were transplanted into nude mice one the harvest size (2.5×2×1 mm3) was reached, it was transplanted onto nude mice. We have developed a cell line from a portion of the DMBA-induced carcinoma of the nude mice. The UHKBR-01 cell exhibited a slow increase in growth rate during the time of culture and was highly tumourigenic in nude mice. The cells have been grown in culture for over 40 passages. Characterization of the cell line was performed. This included morphology by light and transmission electron microscopy, karyotype, growth rate, tumour antigen expression and xenograft implantation into nude mice. These cells exhibit ultrastructural and immunohistochemical features of epithelial cells of mammary origin. The above analyses also demonstrated that UHKBR-01 cells were oestrogen- and progesterone-receptor positive, in likeness to other established breast cancer cell lines such as MDA-MB-231 and MCF-7. The cell line grows as monolayers of oval-shaped cells with large folded nuclei accompanied by a rich supply of mitochondria. This report describes the first in vitro cell line from transplantable DMBA-induced mammary carcinoma of nude mice, which presents unique characteristics that may prove to be a good experimental model for investigating breast cancer biology.

    Original languageEnglish (US)
    Pages (from-to)27-40
    Number of pages14
    JournalLife Sciences
    Volume73
    Issue number1
    DOIs
    StatePublished - May 23 2003

    Keywords

    • DMBA
    • Electron microscopy
    • Karyotyping
    • Oestrogen
    • SD rat

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Chow, L. W. C., Cheung, M. N. B., Loo, W. T. Y., & Guan, X. Y. (2003). A rat cell line derived from DMBA-induced mammary carcinoma. Life Sciences, 73(1), 27-40. https://doi.org/10.1016/S0024-3205(03)00253-4