Ablation of miR-10b suppresses oncogene-induced mammary tumorigenesis and metastasis and reactivates tumor-suppressive pathways

Jongchan Kim, Ashley N. Siverly, Dahu Chen, Min Wang, Yuan Yuan, Yumeng Wang, Hyemin Lee, Jinsong Zhang, William J. Muller, Han Liang, Boyi Gan, Xianbin Yang, Yutong Sun, M. James You, Li Ma

Research output: Contribution to journalArticle

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Abstract

The invasive and metastatic properties of many human tumors have been associated with upregulation of the miRNA miR-10b, but its functional contributions in this setting have not been fully unraveled. Here, we report the generation of miR-10b-deficient mice, in which miR-10b is shown to be largely dispensable for normal development but critical to tumorigenesis. Loss of miR- 10b delays oncogene-induced mammary tumorigenesis and suppresses epithelial-mesenchymal transition, intravasation, and metastasis in a mouse model of metastatic breast cancer. Among the target genes of miR-10b, the tumor suppressor genes Tbx5 and Pten and the metastasis suppressor gene Hoxd10 are significantly upregulated by miR-10b deletion. Mechanistically, miR-10b promotes breast cancer cell proliferation, migration, and invasion through inhibition of the expression of the transcription factor TBX5, leading to repression of the tumor suppressor genes DYRK1A and PTEN. In clinical specimens of breast cancer, the expression of TBX5, HOXD10, and DYRK1A correlates with relapse-free survival and overall survival outcomes in patients. Our results establish miR-10b as an oncomiR that drives metastasis, termed a metastamiR, and define the set of critical tumor suppressor mechanisms it overcomes to drive breast cancer progression.

Original languageEnglish (US)
Pages (from-to)6424-6435
Number of pages12
JournalCancer research
Volume76
Issue number21
DOIs
StatePublished - Nov 1 2016

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Oncogenes
Carcinogenesis
Breast
Tumor Suppressor Genes
Breast Neoplasms
Neoplasm Metastasis
Neoplasms
Epithelial-Mesenchymal Transition
Survival
MicroRNAs
Cell Movement
Transcription Factors
Up-Regulation
Cell Proliferation
Recurrence
Genes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Ablation of miR-10b suppresses oncogene-induced mammary tumorigenesis and metastasis and reactivates tumor-suppressive pathways. / Kim, Jongchan; Siverly, Ashley N.; Chen, Dahu; Wang, Min; Yuan, Yuan; Wang, Yumeng; Lee, Hyemin; Zhang, Jinsong; Muller, William J.; Liang, Han; Gan, Boyi; Yang, Xianbin; Sun, Yutong; You, M. James; Ma, Li.

In: Cancer research, Vol. 76, No. 21, 01.11.2016, p. 6424-6435.

Research output: Contribution to journalArticle

Kim, Jongchan ; Siverly, Ashley N. ; Chen, Dahu ; Wang, Min ; Yuan, Yuan ; Wang, Yumeng ; Lee, Hyemin ; Zhang, Jinsong ; Muller, William J. ; Liang, Han ; Gan, Boyi ; Yang, Xianbin ; Sun, Yutong ; You, M. James ; Ma, Li. / Ablation of miR-10b suppresses oncogene-induced mammary tumorigenesis and metastasis and reactivates tumor-suppressive pathways. In: Cancer research. 2016 ; Vol. 76, No. 21. pp. 6424-6435.
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