Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line

Susan P. Lees-Miller; Roseline Godbout; Doug W. Chan; Michael Weinfeld; Rufus S. Day; Geraldine M. Barron; Joan Allalunis-Turner

Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line

Susan P. Lees-Miller
, Roseline Godbout
, Doug W. Chan
, Michael Weinfeld
, Rufus S. Day
, Geraldine M. Barron
, Joan Allalunis-Turner

Research output: Contribution to journal › Article › peer-review

Abstract

The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.

Original language	English (US)
Pages (from-to)	1183-1185
Number of pages	3
Journal	Science
Volume	267
Issue number	5201
State	Published - Feb 24 1995
Externally published	Yes

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Cite this

@article{e38e2a8d6dce4181b7009a6247187cff,

title = "Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line",

abstract = "The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.",

author = "Lees-Miller, \{Susan P.\} and Roseline Godbout and Chan, \{Doug W.\} and Michael Weinfeld and Day, \{Rufus S.\} and Barron, \{Geraldine M.\} and Joan Allalunis-Turner",

year = "1995",

month = feb,

day = "24",

language = "English (US)",

volume = "267",

pages = "1183--1185",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

number = "5201",

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T1 - Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line

AU - Lees-Miller, Susan P.

AU - Godbout, Roseline

AU - Chan, Doug W.

AU - Weinfeld, Michael

AU - Day, Rufus S.

AU - Barron, Geraldine M.

AU - Allalunis-Turner, Joan

PY - 1995/2/24

Y1 - 1995/2/24

N2 - The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.

AB - The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.

UR - https://www.scopus.com/pages/publications/0028937877

UR - https://www.scopus.com/pages/publications/0028937877#tab=citedBy

M3 - Article

C2 - 7855602

AN - SCOPUS:0028937877

SN - 0036-8075

VL - 267

SP - 1183

EP - 1185

JO - Science

JF - Science

IS - 5201

ER -

Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line

Abstract

ASJC Scopus subject areas

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