Activation of Aurora A kinase increases YAP stability via blockage of autophagy

Peng Wang; Ying Gong; Tao Guo; Man Li; Lei Fang; Shengchen Yin; Muhammad Kamran; Yang Liu; Jie Xu; Lingzhi Xu; Fei Peng; Xiaoyuan Xue; Mengying Yang; Mie Chie Hung; Eric W.F. Lam; Chundong Gu; Chunli Wang; Qimin Zhan; Quentin Liu

doi:10.1038/s41419-019-1664-4

Activation of Aurora A kinase increases YAP stability via blockage of autophagy

Peng Wang, Ying Gong, Tao Guo, Man Li, Lei Fang, Shengchen Yin, Muhammad Kamran, Yang Liu, Jie Xu, Lingzhi Xu, Fei Peng, Xiaoyuan Xue, Mengying Yang, Mie Chie Hung, Eric W.F. Lam, Chundong Gu, Chunli Wang, Qimin Zhan, Quentin Liu

Research output: Contribution to journal › Article › peer-review

40 Scopus citations

Abstract

Transcription cofactor Yes-associated protein (YAP) plays an important role in cancer progression. Here, we found that Aurora A kinase expression was positively correlated with YAP in lung cancer. Aurora A depletion suppresses lung cancer cell colony formation, which could be reversed by YAP ectopic overexpression. In addition, activation of Aurora A increases YAP protein abundance through maintaining its protein stability. Consistently, the transcriptional activity of YAP is increased upon Aurora A activation. We further showed that shAURKA suppressed YAP expression in the absence of Lats1/2, indicating that Aurora A regulates YAP independently of Hippo pathway. Instead, Aurora A induced blockage of autophagy to up-regulate YAP expression. Collectively, our findings provide insights into regulatory mechanisms of YAP expression in lung cancer development.

Original language	English (US)
Article number	432
Journal	Cell Death and Disease
Volume	10
Issue number	6
DOIs	https://doi.org/10.1038/s41419-019-1664-4
State	Published - Jun 1 2019

ASJC Scopus subject areas

Immunology
Cellular and Molecular Neuroscience
Cell Biology
Cancer Research

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title = "Activation of Aurora A kinase increases YAP stability via blockage of autophagy",

abstract = "Transcription cofactor Yes-associated protein (YAP) plays an important role in cancer progression. Here, we found that Aurora A kinase expression was positively correlated with YAP in lung cancer. Aurora A depletion suppresses lung cancer cell colony formation, which could be reversed by YAP ectopic overexpression. In addition, activation of Aurora A increases YAP protein abundance through maintaining its protein stability. Consistently, the transcriptional activity of YAP is increased upon Aurora A activation. We further showed that shAURKA suppressed YAP expression in the absence of Lats1/2, indicating that Aurora A regulates YAP independently of Hippo pathway. Instead, Aurora A induced blockage of autophagy to up-regulate YAP expression. Collectively, our findings provide insights into regulatory mechanisms of YAP expression in lung cancer development.",

author = "Peng Wang and Ying Gong and Tao Guo and Man Li and Lei Fang and Shengchen Yin and Muhammad Kamran and Yang Liu and Jie Xu and Lingzhi Xu and Fei Peng and Xiaoyuan Xue and Mengying Yang and Hung, {Mie Chie} and Lam, {Eric W.F.} and Chundong Gu and Chunli Wang and Qimin Zhan and Quentin Liu",

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doi = "10.1038/s41419-019-1664-4",

language = "English (US)",

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T1 - Activation of Aurora A kinase increases YAP stability via blockage of autophagy

AU - Wang, Peng

AU - Gong, Ying

AU - Guo, Tao

AU - Li, Man

AU - Fang, Lei

AU - Yin, Shengchen

AU - Kamran, Muhammad

AU - Liu, Yang

AU - Xu, Jie

AU - Xu, Lingzhi

AU - Peng, Fei

AU - Xue, Xiaoyuan

AU - Yang, Mengying

AU - Hung, Mie Chie

AU - Lam, Eric W.F.

AU - Gu, Chundong

AU - Wang, Chunli

AU - Zhan, Qimin

AU - Liu, Quentin

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Transcription cofactor Yes-associated protein (YAP) plays an important role in cancer progression. Here, we found that Aurora A kinase expression was positively correlated with YAP in lung cancer. Aurora A depletion suppresses lung cancer cell colony formation, which could be reversed by YAP ectopic overexpression. In addition, activation of Aurora A increases YAP protein abundance through maintaining its protein stability. Consistently, the transcriptional activity of YAP is increased upon Aurora A activation. We further showed that shAURKA suppressed YAP expression in the absence of Lats1/2, indicating that Aurora A regulates YAP independently of Hippo pathway. Instead, Aurora A induced blockage of autophagy to up-regulate YAP expression. Collectively, our findings provide insights into regulatory mechanisms of YAP expression in lung cancer development.

AB - Transcription cofactor Yes-associated protein (YAP) plays an important role in cancer progression. Here, we found that Aurora A kinase expression was positively correlated with YAP in lung cancer. Aurora A depletion suppresses lung cancer cell colony formation, which could be reversed by YAP ectopic overexpression. In addition, activation of Aurora A increases YAP protein abundance through maintaining its protein stability. Consistently, the transcriptional activity of YAP is increased upon Aurora A activation. We further showed that shAURKA suppressed YAP expression in the absence of Lats1/2, indicating that Aurora A regulates YAP independently of Hippo pathway. Instead, Aurora A induced blockage of autophagy to up-regulate YAP expression. Collectively, our findings provide insights into regulatory mechanisms of YAP expression in lung cancer development.

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JO - Cell Death and Disease

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Activation of Aurora A kinase increases YAP stability via blockage of autophagy

Abstract

ASJC Scopus subject areas

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