Activation of p53 Gene Expression in Premalignant Lesions during Head and Neck Ttomorigenesis

Dong M. Shin, Waun K. Hong, Jhingook Kim, Jack A. Roth, Thoracic Surgery, Jae Y. Ro, Jason Hittelman, Walter N. Hittelman

Research output: Contribution to journalArticle

268 Citations (Scopus)

Abstract

With the goal of identifying a potential intermediate biomarker in the multistep process of head and neck cancer development, we conducted immunohistochemical analyses for p53 expression in 33 patients with head and neck squamous cell carcinomas whose tissue sections contained adjacent normal epithelium, hyperplastic, and/or dysplastic lesions. Fifteen of 33 (45%) squamous cell carcinomas of the head and neck expressed p53, but none of four normal control patients (cancer-free nonsmokers) expressed detectable p53 in oral mucosa specimens. To determine when p53 expression is initiated during head and neck tumorigenesis, we examined the normal and premalignant lesions adjacent to the tumors. Five of 24 (21%) samples of normal epithelium adjacent to tumors, 7 of 24 (29%) samples of hyperplasia, and 9 of 20 (45%) samples of dysplasia expressed p53. Quantitative image analysis demonstrated not only a gradual increase in the amount of p53 expression as tissue abnormalities progressed but also a topological change in expression. Whereas p53 expression, when present, was limited to the basal layer in normal epithelium adjacent to tumor, the expression of p53 expanded into the parabasal and superficial layers in hyperplasia and dysplasia. We conclude that p53 expression can be altered in very early phases of head and neck tumorigenesis. Thus, it may be an excellent candidate for risk assessment and may serve as an intermediate biomarker in chemoprevention trials.

Original languageEnglish (US)
Pages (from-to)321-326
Number of pages6
JournalCancer research
Volume54
Issue number2
StatePublished - Jan 1994

Fingerprint

p53 Genes
Neck
Head
Gene Expression
Epithelium
Hyperplasia
Neoplasms
Carcinogenesis
Biomarkers
Chemoprevention
Mouth Mucosa
Head and Neck Neoplasms
Carcinoma, squamous cell of head and neck

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Shin, D. M., Hong, W. K., Kim, J., Roth, J. A., Surgery, T., Ro, J. Y., ... Hittelman, W. N. (1994). Activation of p53 Gene Expression in Premalignant Lesions during Head and Neck Ttomorigenesis. Cancer research, 54(2), 321-326.

Activation of p53 Gene Expression in Premalignant Lesions during Head and Neck Ttomorigenesis. / Shin, Dong M.; Hong, Waun K.; Kim, Jhingook; Roth, Jack A.; Surgery, Thoracic; Ro, Jae Y.; Hittelman, Jason; Hittelman, Walter N.

In: Cancer research, Vol. 54, No. 2, 01.1994, p. 321-326.

Research output: Contribution to journalArticle

Shin, DM, Hong, WK, Kim, J, Roth, JA, Surgery, T, Ro, JY, Hittelman, J & Hittelman, WN 1994, 'Activation of p53 Gene Expression in Premalignant Lesions during Head and Neck Ttomorigenesis', Cancer research, vol. 54, no. 2, pp. 321-326.
Shin, Dong M. ; Hong, Waun K. ; Kim, Jhingook ; Roth, Jack A. ; Surgery, Thoracic ; Ro, Jae Y. ; Hittelman, Jason ; Hittelman, Walter N. / Activation of p53 Gene Expression in Premalignant Lesions during Head and Neck Ttomorigenesis. In: Cancer research. 1994 ; Vol. 54, No. 2. pp. 321-326.
@article{1efdc7c59dfb43c5a0af13f81b7370fb,
title = "Activation of p53 Gene Expression in Premalignant Lesions during Head and Neck Ttomorigenesis",
abstract = "With the goal of identifying a potential intermediate biomarker in the multistep process of head and neck cancer development, we conducted immunohistochemical analyses for p53 expression in 33 patients with head and neck squamous cell carcinomas whose tissue sections contained adjacent normal epithelium, hyperplastic, and/or dysplastic lesions. Fifteen of 33 (45{\%}) squamous cell carcinomas of the head and neck expressed p53, but none of four normal control patients (cancer-free nonsmokers) expressed detectable p53 in oral mucosa specimens. To determine when p53 expression is initiated during head and neck tumorigenesis, we examined the normal and premalignant lesions adjacent to the tumors. Five of 24 (21{\%}) samples of normal epithelium adjacent to tumors, 7 of 24 (29{\%}) samples of hyperplasia, and 9 of 20 (45{\%}) samples of dysplasia expressed p53. Quantitative image analysis demonstrated not only a gradual increase in the amount of p53 expression as tissue abnormalities progressed but also a topological change in expression. Whereas p53 expression, when present, was limited to the basal layer in normal epithelium adjacent to tumor, the expression of p53 expanded into the parabasal and superficial layers in hyperplasia and dysplasia. We conclude that p53 expression can be altered in very early phases of head and neck tumorigenesis. Thus, it may be an excellent candidate for risk assessment and may serve as an intermediate biomarker in chemoprevention trials.",
author = "Shin, {Dong M.} and Hong, {Waun K.} and Jhingook Kim and Roth, {Jack A.} and Thoracic Surgery and Ro, {Jae Y.} and Jason Hittelman and Hittelman, {Walter N.}",
year = "1994",
month = "1",
language = "English (US)",
volume = "54",
pages = "321--326",
journal = "Cancer Research",
issn = "0008-5472",
publisher = "American Association for Cancer Research Inc.",
number = "2",

}

TY - JOUR

T1 - Activation of p53 Gene Expression in Premalignant Lesions during Head and Neck Ttomorigenesis

AU - Shin, Dong M.

AU - Hong, Waun K.

AU - Kim, Jhingook

AU - Roth, Jack A.

AU - Surgery, Thoracic

AU - Ro, Jae Y.

AU - Hittelman, Jason

AU - Hittelman, Walter N.

PY - 1994/1

Y1 - 1994/1

N2 - With the goal of identifying a potential intermediate biomarker in the multistep process of head and neck cancer development, we conducted immunohistochemical analyses for p53 expression in 33 patients with head and neck squamous cell carcinomas whose tissue sections contained adjacent normal epithelium, hyperplastic, and/or dysplastic lesions. Fifteen of 33 (45%) squamous cell carcinomas of the head and neck expressed p53, but none of four normal control patients (cancer-free nonsmokers) expressed detectable p53 in oral mucosa specimens. To determine when p53 expression is initiated during head and neck tumorigenesis, we examined the normal and premalignant lesions adjacent to the tumors. Five of 24 (21%) samples of normal epithelium adjacent to tumors, 7 of 24 (29%) samples of hyperplasia, and 9 of 20 (45%) samples of dysplasia expressed p53. Quantitative image analysis demonstrated not only a gradual increase in the amount of p53 expression as tissue abnormalities progressed but also a topological change in expression. Whereas p53 expression, when present, was limited to the basal layer in normal epithelium adjacent to tumor, the expression of p53 expanded into the parabasal and superficial layers in hyperplasia and dysplasia. We conclude that p53 expression can be altered in very early phases of head and neck tumorigenesis. Thus, it may be an excellent candidate for risk assessment and may serve as an intermediate biomarker in chemoprevention trials.

AB - With the goal of identifying a potential intermediate biomarker in the multistep process of head and neck cancer development, we conducted immunohistochemical analyses for p53 expression in 33 patients with head and neck squamous cell carcinomas whose tissue sections contained adjacent normal epithelium, hyperplastic, and/or dysplastic lesions. Fifteen of 33 (45%) squamous cell carcinomas of the head and neck expressed p53, but none of four normal control patients (cancer-free nonsmokers) expressed detectable p53 in oral mucosa specimens. To determine when p53 expression is initiated during head and neck tumorigenesis, we examined the normal and premalignant lesions adjacent to the tumors. Five of 24 (21%) samples of normal epithelium adjacent to tumors, 7 of 24 (29%) samples of hyperplasia, and 9 of 20 (45%) samples of dysplasia expressed p53. Quantitative image analysis demonstrated not only a gradual increase in the amount of p53 expression as tissue abnormalities progressed but also a topological change in expression. Whereas p53 expression, when present, was limited to the basal layer in normal epithelium adjacent to tumor, the expression of p53 expanded into the parabasal and superficial layers in hyperplasia and dysplasia. We conclude that p53 expression can be altered in very early phases of head and neck tumorigenesis. Thus, it may be an excellent candidate for risk assessment and may serve as an intermediate biomarker in chemoprevention trials.

UR - http://www.scopus.com/inward/record.url?scp=0028158007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028158007&partnerID=8YFLogxK

M3 - Article

C2 - 8275461

AN - SCOPUS:0028158007

VL - 54

SP - 321

EP - 326

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 2

ER -