Adenovirus-mediated transfer of a wild-type p53 gene and induction of apoptosis in cervical cancer

Katsuyuki Hamada, Ramon Alemany, Wei Wei Zhang, Walter N. Hittelman, Reuben Lotan, Jack A Roth, Michele Follen Mitchell

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

In most cervical cancers, the function of p53 is down regulated. To explore the potential use of p53 in gene therapy for cervical cancer, we introduced wild-type p53 into cervical cancer cell lines via a recombinant adenoviral vector, Ad5CMV-p53, and analyzed its effects on cell and tumor growth. The transduction efficiencies of all cell lines were 100% at a multiplicity of infection of 100 or greater. The p53 protein was detected in Ad5CMV-p53-infected cells. Protein expression peaked at day 3 after infection and lasted 15 days. The Ad5CMV-p53-infected cells underwent apoptosis, and cell growth was greatly suppressed. The Ad5CMV-p53 treatment significantly reduced the volumes of established s.c. tumors in vivo. These results indicate that transfection of cervical cancer cells with the wild-type p53 gene via Ad5CMV-p53 is a potential novel approach to the therapy of cervical cancer.

Original languageEnglish (US)
Pages (from-to)3047-3054
Number of pages8
JournalCancer Research
Volume56
Issue number13
StatePublished - Jul 1 1996

Fingerprint

p53 Genes
Adenoviridae
Uterine Cervical Neoplasms
Apoptosis
Cell Line
Growth
Infection
Genetic Therapy
Transfection
Neoplasms
Proteins
Therapeutics

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Hamada, K., Alemany, R., Zhang, W. W., Hittelman, W. N., Lotan, R., Roth, J. A., & Mitchell, M. F. (1996). Adenovirus-mediated transfer of a wild-type p53 gene and induction of apoptosis in cervical cancer. Cancer Research, 56(13), 3047-3054.

Adenovirus-mediated transfer of a wild-type p53 gene and induction of apoptosis in cervical cancer. / Hamada, Katsuyuki; Alemany, Ramon; Zhang, Wei Wei; Hittelman, Walter N.; Lotan, Reuben; Roth, Jack A; Mitchell, Michele Follen.

In: Cancer Research, Vol. 56, No. 13, 01.07.1996, p. 3047-3054.

Research output: Contribution to journalArticle

Hamada, K, Alemany, R, Zhang, WW, Hittelman, WN, Lotan, R, Roth, JA & Mitchell, MF 1996, 'Adenovirus-mediated transfer of a wild-type p53 gene and induction of apoptosis in cervical cancer', Cancer Research, vol. 56, no. 13, pp. 3047-3054.
Hamada K, Alemany R, Zhang WW, Hittelman WN, Lotan R, Roth JA et al. Adenovirus-mediated transfer of a wild-type p53 gene and induction of apoptosis in cervical cancer. Cancer Research. 1996 Jul 1;56(13):3047-3054.
Hamada, Katsuyuki ; Alemany, Ramon ; Zhang, Wei Wei ; Hittelman, Walter N. ; Lotan, Reuben ; Roth, Jack A ; Mitchell, Michele Follen. / Adenovirus-mediated transfer of a wild-type p53 gene and induction of apoptosis in cervical cancer. In: Cancer Research. 1996 ; Vol. 56, No. 13. pp. 3047-3054.
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