Adiponectin regulates expression of hepatic genes critical for glucose and lipid metabolism

Qingqing Liu, Bingbing Yuan, Kinyui Alice Lo, Heide Christine Patterson, Yutong Sun, Harvey F. Lodish

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The effects of adiponectin on hepatic glucose and lipid metabolism at transcriptional level are largely unknown. We profiled hepatic gene expression in adiponectin knockout (KO) and wild-type (WT) mice by RNA sequencing. Compared with WT mice, adiponectin KO mice fed a chow diet exhibited decreased mRNA expression of rate-limiting enzymes in several important glucose and lipid metabolic pathways, including glycolysis, tricarboxylic acid cycle, fatty-acid activation and synthesis, triglyceride synthesis, and cholesterol synthesis. In addition, binding of the transcription factor Hnf4a to DNAs encoding several key metabolic enzymes was reduced in KO mice, suggesting that adiponectin might regulate hepatic gene expression via Hnf4a. Phenotypically, adiponectin KO mice possessed smaller epididymal fat pads and showed reduced body weight compared with WT mice. When fed a high-fat diet, adiponectin KO mice showed significantly reduced lipid accumulation in the liver. These lipogenic defects are consistent with the down-regulation of lipogenic genes in the KO mice.

Original languageEnglish (US)
Pages (from-to)14568-14573
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number36
DOIs
StatePublished - Sep 4 2012

Fingerprint

Adiponectin
Lipid Metabolism
Knockout Mice
Gene Expression
Glucose
Liver
RNA Sequence Analysis
Lipids
Gene Knockout Techniques
Citric Acid Cycle
High Fat Diet
Glycolysis
Enzymes
Metabolic Networks and Pathways
Adipose Tissue
Triglycerides
Transcription Factors
Fatty Acids
Down-Regulation
Cholesterol

Keywords

  • Hepatic metabolism
  • Lipogenesis
  • Transcriptional regulation

ASJC Scopus subject areas

  • General

Cite this

Adiponectin regulates expression of hepatic genes critical for glucose and lipid metabolism. / Liu, Qingqing; Yuan, Bingbing; Lo, Kinyui Alice; Patterson, Heide Christine; Sun, Yutong; Lodish, Harvey F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, No. 36, 04.09.2012, p. 14568-14573.

Research output: Contribution to journalArticle

Liu, Qingqing ; Yuan, Bingbing ; Lo, Kinyui Alice ; Patterson, Heide Christine ; Sun, Yutong ; Lodish, Harvey F. / Adiponectin regulates expression of hepatic genes critical for glucose and lipid metabolism. In: Proceedings of the National Academy of Sciences of the United States of America. 2012 ; Vol. 109, No. 36. pp. 14568-14573.
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