AFLP analysis of Trichoderma spp. from India compared with sequence and morphological-based diagnostics

H. K. Buhariwalla, P. Srilakshmi, S. Kannan, R. S. Kanchi, S. Chandra, K. Satyaprasad, F. Waliyar, R. P. Thakur, J. H. Crouch

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Trichoderma species offer considerable potential for controlling aflatoxin contamination in groundnut and other crops. Initial classification of 48 Trichoderma isolates, derived from four different groundnut cultivation sites in India was based on alignment of 28S rDNA sequences to GenBank sequences of ex-type strains. This was found to be substantially more reliable than our routine morphological characterization, but did not provide a comprehensive diagnostic solution, as unique single nucleotide polymorphism (SNP) haplotypes could not be identified for all species. However, all the Trichoderma isolates could be readily distinguished by amplified fragment length polymorphism (AFLP) analysis, based on six primer pair combinations, which generated 234 polymorphic bands. In addition, individual AFLP bands were identified which differentiate closely related species. Similarly, AFLP bands were identified that correlated with different types of antagonism to Aspergillus flavus. The implications of these results for the development of simple polymerase chain reaction (PCR)-based diagnostic assays for antagonistic isolates of Trichoderma is discussed.

Original languageEnglish (US)
Pages (from-to)389-400
Number of pages12
JournalJournal of Phytopathology
Volume153
Issue number7-8
DOIs
StatePublished - 2005

Keywords

  • Aflatoxin
  • Amplified fragment length polymorphism
  • Groundnut
  • Internal transcribed spacer
  • Trichoderma

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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