AFM to study pore-forming proteins

Joseph D. Unsay, Ana Garcia-Saez

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Atomic force microscopy (AFM) is a form of contact microscopy that uses a very sharp tip to scan the surface of a sample. It provides a 3D image of the surface structure and in the force mode it can also be used to test the mechanical properties of the sample. AFM has been successfully applied to study the molecular mechanism of pore-forming proteins on model membranes. It gives information about both the structural reorganization of the membrane surface and the changes in the force required for membrane piercing upon incubation with this special type of proteins. Here we describe robust protocols to investigate the effect of pore-forming proteins in supported lipid bilayers.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages191-202
Number of pages12
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1886
ISSN (Print)1064-3745

Fingerprint

Porins
Atomic Force Microscopy
Membranes
Lipid Bilayers
Microscopy
Proteins

Keywords

  • Atomic force microscopy
  • Bax
  • Piercing force
  • Pore-forming protein
  • Supported lipid bilayer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Unsay, J. D., & Garcia-Saez, A. (2019). AFM to study pore-forming proteins. In Methods in Molecular Biology (pp. 191-202). (Methods in Molecular Biology; Vol. 1886). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8894-5_10

AFM to study pore-forming proteins. / Unsay, Joseph D.; Garcia-Saez, Ana.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 191-202 (Methods in Molecular Biology; Vol. 1886).

Research output: Chapter in Book/Report/Conference proceedingChapter

Unsay, JD & Garcia-Saez, A 2019, AFM to study pore-forming proteins. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1886, Humana Press Inc., pp. 191-202. https://doi.org/10.1007/978-1-4939-8894-5_10
Unsay JD, Garcia-Saez A. AFM to study pore-forming proteins. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 191-202. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8894-5_10
Unsay, Joseph D. ; Garcia-Saez, Ana. / AFM to study pore-forming proteins. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 191-202 (Methods in Molecular Biology).
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