TY - JOUR
T1 - Age-related long-term response in rat thyroid tissue and plasma after internal low dose exposure to 131I
AU - Larsson, Malin
AU - Rudqvist, Nils Petter
AU - Spetz, Johan
AU - Parris, Toshima Z.
AU - Langen, Britta
AU - Helou, Khalil
AU - Forssell-Aronsson, Eva
N1 - Funding Information:
The authors would like to thank Lab techs Lilian Karlsson and Ann Wikström for their skilled technical assistance, and Daniella Pettersson for ELISA analyses. We thank the Core facility at Novum, BEA, Bioinformatics and Expression Analysis, which is supported by the board of research at the Karolinska Institute and the research committee at the Karolinska University Hospital. Quantitative proteomic analysis was performed at the Proteomics Core Facility at Sahlgrenska Academy, University of Gothenburg. The Proteomics Core Facility is grateful to the Inga-Britt and Arne Lundberg Research Foundation for the donation of the Orbitrap Fusion Tribrid MS instrument.
Funding Information:
Open access funding provided by University of Gothenburg. This study was supported by Grants from BioCARE—a National Strategic Research Program at the University of Gothenburg, the Swedish Cancer Society (Grant no. 3427), the Swedish Research Council (Grant no. 21073), the Swedish state under the agreement between the Swedish government and the county councils—the ALF-agreement (ALFGBG-725031), Swedish Radiation Safety Authority (SSM), the King Gustav V Jubilee Clinic Cancer Research Foundation, the Sahlgrenska University Hospital Research Funds, the Assar Gabrielsson Cancer Research Foundation, the Adlerbertska Research Foundation, the Knut and Alice Wallenberg Foundation, the Royal Society of Arts and Sciences in Gothenburg (KVVS), and the Wilhelm and Martina Lundgren Research Foundation.
Funding Information:
The authors would like to thank Lab techs Lilian Karlsson and Ann Wikstr?m for their skilled technical assistance, and Daniella Pettersson for ELISA analyses. We thank the Core facility at Novum, BEA, Bioinformatics and Expression Analysis, which is supported by the board of research at the Karolinska Institute and the research committee at the Karolinska University Hospital. Quantitative proteomic analysis was performed at the Proteomics Core Facility at Sahlgrenska Academy, University of Gothenburg. The Proteomics Core Facility is grateful to the Inga-Britt and Arne Lundberg Research Foundation for the donation of the Orbitrap Fusion Tribrid MS instrument.
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - 131I is used clinically for therapy, and may be released during nuclear accidents. After the Chernobyl accident papillary thyroid carcinoma incidence increased in children, but not adults. The aims of this study were to compare 131I irradiation-dependent differences in RNA and protein expression in the thyroid and plasma of young and adult rats, and identify potential age-dependent biomarkers for 131I exposure. Twelve young (5 weeks) and twelve adult Sprague Dawley rats (17 weeks) were i.v. injected with 50 kBq 131I (absorbed dose to thyroid = 0.1 Gy), and sixteen unexposed age-matched rats were used as controls. The rats were killed 3–9 months after administration. Microarray analysis was performed using RNA from thyroid samples, while LC–MS/MS analysis was performed on proteins extracted from thyroid tissue and plasma. Canonical pathways, biological functions and upstream regulators were analysed for the identified transcripts and proteins. Distinct age-dependent differences in gene and protein expression were observed. Novel biomarkers for thyroid 131I exposure were identified: (PTH), age-dependent dose response (CA1, FTL1, PVALB (youngsters) and HSPB6 (adults)), thyroid function (Vegfb (adults)). Further validation using clinical samples are needed to explore the role of the identified biomarkers.
AB - 131I is used clinically for therapy, and may be released during nuclear accidents. After the Chernobyl accident papillary thyroid carcinoma incidence increased in children, but not adults. The aims of this study were to compare 131I irradiation-dependent differences in RNA and protein expression in the thyroid and plasma of young and adult rats, and identify potential age-dependent biomarkers for 131I exposure. Twelve young (5 weeks) and twelve adult Sprague Dawley rats (17 weeks) were i.v. injected with 50 kBq 131I (absorbed dose to thyroid = 0.1 Gy), and sixteen unexposed age-matched rats were used as controls. The rats were killed 3–9 months after administration. Microarray analysis was performed using RNA from thyroid samples, while LC–MS/MS analysis was performed on proteins extracted from thyroid tissue and plasma. Canonical pathways, biological functions and upstream regulators were analysed for the identified transcripts and proteins. Distinct age-dependent differences in gene and protein expression were observed. Novel biomarkers for thyroid 131I exposure were identified: (PTH), age-dependent dose response (CA1, FTL1, PVALB (youngsters) and HSPB6 (adults)), thyroid function (Vegfb (adults)). Further validation using clinical samples are needed to explore the role of the identified biomarkers.
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UR - http://www.scopus.com/inward/citedby.url?scp=85124327938&partnerID=8YFLogxK
U2 - 10.1038/s41598-022-06071-4
DO - 10.1038/s41598-022-06071-4
M3 - Article
C2 - 35136135
AN - SCOPUS:85124327938
SN - 2045-2322
VL - 12
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 2107
ER -