ALPK1 Expression Is Associated with Lymph Node Metastasis and Tumor Growth in Oral Squamous Cell Carcinoma Patients

Po Ku Chen, Chun Hung Hua, Hui Ting Hsu, Tzer Min Kuo, Chia Min Chung, Chi Pin Lee, Ming-Hsui Tsai, Kun Tu Yeh, Ying Chin Ko

Research output: Contribution to journalArticle

Abstract

Oral squamous cell carcinoma (OSCC) is the most common malignant cancer, with high mortality rates in advanced stages. Recent studies have shown that the expression of ALPK1 mRNA and its inhibitory differentiation function are associated with cancer progression. However, the expression and clinicopathologic features of ALPK1 in OSCC remain unexplored. Herein, the authors investigated the expression patterns of ALPK1 in 39 matched OSCC patients and examined the relationship between ALPK1 protein expression and clinicopathologic factors using immunohistochemical scores. Using Western blot analysis, ALPK1 expression was found to be significantly higher in tumor tissues than that in nontumor tissues. Through an immunoreactive scoring system, a significantly higher number of advanced-stage tumor size T4 and lymph node metastasis N2 exhibited higher ALPK1 expression levels than that exhibited by T1/T2/T3 tumors and N0/N1. In addition, ALPK1 protein expression was aberrant in malignant oral cancer cell lines compared with that in pre-malignant oral epithelial cells, whereas minimal expression was observed in normal oral epithelial cells. Knockdown of ALPK1 resulted in a significant reduction in cell growth, migration, and invasion capacity in vitro. Consequently, expression of N-cadherin and vimentin decreased in ALPK1-deficient cells. Thus, these results suggest that ALPK1 serves as a potential biomarker and target for OSCC development in late stages.

Original languageEnglish (US)
Pages (from-to)190-199
Number of pages10
JournalAmerican Journal of Pathology
Volume189
Issue number1
DOIs
StatePublished - Jan 1 2019

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Squamous Cell Carcinoma
Lymph Nodes
Neoplasm Metastasis
Growth
Neoplasms
Epithelial Cells
Mouth Neoplasms
Vimentin
Cadherins
Cell Movement
Proteins
Biomarkers
Western Blotting
Cell Line
Messenger RNA
Mortality

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

ALPK1 Expression Is Associated with Lymph Node Metastasis and Tumor Growth in Oral Squamous Cell Carcinoma Patients. / Chen, Po Ku; Hua, Chun Hung; Hsu, Hui Ting; Kuo, Tzer Min; Chung, Chia Min; Lee, Chi Pin; Tsai, Ming-Hsui; Yeh, Kun Tu; Ko, Ying Chin.

In: American Journal of Pathology, Vol. 189, No. 1, 01.01.2019, p. 190-199.

Research output: Contribution to journalArticle

Chen, Po Ku ; Hua, Chun Hung ; Hsu, Hui Ting ; Kuo, Tzer Min ; Chung, Chia Min ; Lee, Chi Pin ; Tsai, Ming-Hsui ; Yeh, Kun Tu ; Ko, Ying Chin. / ALPK1 Expression Is Associated with Lymph Node Metastasis and Tumor Growth in Oral Squamous Cell Carcinoma Patients. In: American Journal of Pathology. 2019 ; Vol. 189, No. 1. pp. 190-199.
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