Alterations of p53, cyclin D1, Rb, and H-ras in human oral carcinomas related to tobacco use

Jie Xu, Irma B. Gimenez-Conti, Joan E. Cunningham, Ana M. Collet, Mario A. Luna, Hector E. Lanfranchi, Margaret R. Spitz, Claudio J. Conti

Research output: Contribution to journalArticle

101 Scopus citations

Abstract

BACKGROUND. Epidemiologic studies have indicated that environmental and personal habits, particularly tobacco use and alcohol abuse, are major etiologic factors in the induction and progression of head and neck squamous cell carcinomas (HNSCC). Molecular studies have focused on HNSCC related to smoking but not those associated with smokeless tobacco. METHODS. The authors studied immunohistochemical evidence of alterations of p53, cyclin D1, and Rb in 34 human oral carcinomas related to tobacco use. They also examined p53 and H-ras using single strand conformation polymorphism (SSCP) and sequencing analysis. RESULTS. Overexpression of cyclin D1 was found in 41% of cases, and accumulation of p53 was found in 59%. Only 9% of the samples did not show Rb staining. In SSCP and sequencing analysis, 17 cases showed mutations in the conserved region of the p53 gene. No mutations were detected in codons 12, 13, or 61 of the H-ras gene. CONCLUSIONS. Overexpression of cyclin D1 and p53 mutations are common alterations in HNSCC. In contrast, the loss of Rb function seems to occur infrequently, and mutations in the H-ras gene apparently do not play a role in this cancer. HNSCC associated with smokeless tobacco contained the same alterations as those related to smoking.

Original languageEnglish (US)
Pages (from-to)204-212
Number of pages9
JournalCancer
Volume83
Issue number2
DOIs
StatePublished - Jul 15 1998

Keywords

  • Cyclin
  • Head and neck
  • P53
  • Rb; H- ras
  • Smokeless tobacco
  • Smoking
  • Squamous cell carcinoma
  • Tobacco

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Xu, J., Gimenez-Conti, I. B., Cunningham, J. E., Collet, A. M., Luna, M. A., Lanfranchi, H. E., Spitz, M. R., & Conti, C. J. (1998). Alterations of p53, cyclin D1, Rb, and H-ras in human oral carcinomas related to tobacco use. Cancer, 83(2), 204-212. https://doi.org/10.1002/(SICI)1097-0142(19980715)83:2<204::AID-CNCR2>3.0.CO;2-Q