TY - JOUR
T1 - Amide proton transfer imaging allows detection of glioma grades and tumor proliferation
T2 - Comparison with Ki-67 expression and proton MR spectroscopy imaging
AU - Su, C.
AU - Liu, C.
AU - Zhao, L.
AU - Jiang, J.
AU - Zhang, J.
AU - Li, S.
AU - Zhu, W.
AU - Wang, J.
N1 - Funding Information:
Received December 17, 2016; accepted after revision May 7, 2017. From the Department of Radiology (C.S., C.L., L.Z., J.J., J.Z., S.L., W.Z.), Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Han-kou, Wuhan, People’s Republic of China; and Department of Radiation Physics (J.W.), The University of Texas MD Anderson Cancer Center, Houston, Texas. This work was supported by grants from the National Program of the Ministry of Science and Technology of China during the “12th Five-Year Plan” (ID: 2011BAI08B10) and the National Natural Science Foundation of China (No. 81171308 and No. 81570462). Please address correspondence to Wenzhen Zhu, MD, Department of Radiology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, No. 1095 JieFang Ave, Hankou, Wuhan, 430030 PR China; e-mail: zhuwenzhen8612@163.com
PY - 2017/9/1
Y1 - 2017/9/1
N2 - BACKGROUND AND PURPOSE: Prognosis in glioma depends strongly on tumor grade and proliferation. In this prospective study of patients with untreated primary cerebral gliomas,weinvestigated whether amide proton transfer-weighted imaging could reveal tumor proliferation and reliably distinguish low-grade from high-grade gliomas compared with Ki-67 expression and proton MR spectroscopy imaging. MATERIALS AND METHODS: This study included 42 patients with low-grade (n = 28) or high-grade (n = 14) glioma, all of whom underwent conventional MR imaging, proton MR spectroscopy imaging, and amide proton transfer-weighted imaging on the same 3T scanner within 2 weeks before surgery. We assessed metabolites of choline and N-acetylaspartate from proton MR spectroscopy imaging and the asymmetric magnetization transfer ratio at 3.5 ppm from amide proton transfer-weighted imaging and compared them with histopathologic grade and immunohistochemical expression of the proliferation marker Ki-67 in the resected specimens. RESULTS: The asymmetric magnetization transfer ratio at 3.5 ppm values measured by different readers showed good concordance and were significantly higher in high-grade gliomas than in low-grade gliomas (3.61%±0.155 versus 2.64%±0.185, P=.0016), with sensitivity and specificity values of 92.9% and 71.4%, respectively, at a cutoff value of 2.93%. The asymmetric magnetization transfer ratio at 3.5 ppm values correlated with tumor grade (r = 0.506, P = .0006) and Ki-67 labeling index (r = 0.502, P = .002). For all patients, the asymmetric magnetization transfer ratio at 3.5 ppm correlated positively with choline (r=0.43, P=.009) and choline/N-acetylaspartate ratio (r=0.42, P = .01) and negatively with N-acetylaspartate (r=-0.455, P = .005). These correlations held for patients with low-grade gliomas versus those with high-grade gliomas, but the correlation coefficients were higher in high-grade gliomas (choline: r = 0.547, P = .053; N-acetylaspartate: r=-0.644, P = .017; choline/N-acetylaspartate: r = 0.583, P =.036). CONCLUSIONS: The asymmetric magnetization transfer ratio at 3.5 ppm may serve as a potential biomarker not only for assessing proliferation, but also for predicting histopathologic grades in gliomas.
AB - BACKGROUND AND PURPOSE: Prognosis in glioma depends strongly on tumor grade and proliferation. In this prospective study of patients with untreated primary cerebral gliomas,weinvestigated whether amide proton transfer-weighted imaging could reveal tumor proliferation and reliably distinguish low-grade from high-grade gliomas compared with Ki-67 expression and proton MR spectroscopy imaging. MATERIALS AND METHODS: This study included 42 patients with low-grade (n = 28) or high-grade (n = 14) glioma, all of whom underwent conventional MR imaging, proton MR spectroscopy imaging, and amide proton transfer-weighted imaging on the same 3T scanner within 2 weeks before surgery. We assessed metabolites of choline and N-acetylaspartate from proton MR spectroscopy imaging and the asymmetric magnetization transfer ratio at 3.5 ppm from amide proton transfer-weighted imaging and compared them with histopathologic grade and immunohistochemical expression of the proliferation marker Ki-67 in the resected specimens. RESULTS: The asymmetric magnetization transfer ratio at 3.5 ppm values measured by different readers showed good concordance and were significantly higher in high-grade gliomas than in low-grade gliomas (3.61%±0.155 versus 2.64%±0.185, P=.0016), with sensitivity and specificity values of 92.9% and 71.4%, respectively, at a cutoff value of 2.93%. The asymmetric magnetization transfer ratio at 3.5 ppm values correlated with tumor grade (r = 0.506, P = .0006) and Ki-67 labeling index (r = 0.502, P = .002). For all patients, the asymmetric magnetization transfer ratio at 3.5 ppm correlated positively with choline (r=0.43, P=.009) and choline/N-acetylaspartate ratio (r=0.42, P = .01) and negatively with N-acetylaspartate (r=-0.455, P = .005). These correlations held for patients with low-grade gliomas versus those with high-grade gliomas, but the correlation coefficients were higher in high-grade gliomas (choline: r = 0.547, P = .053; N-acetylaspartate: r=-0.644, P = .017; choline/N-acetylaspartate: r = 0.583, P =.036). CONCLUSIONS: The asymmetric magnetization transfer ratio at 3.5 ppm may serve as a potential biomarker not only for assessing proliferation, but also for predicting histopathologic grades in gliomas.
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U2 - 10.3174/ajnr.A5301
DO - 10.3174/ajnr.A5301
M3 - Article
C2 - 28729292
AN - SCOPUS:85029218901
SN - 0195-6108
VL - 38
SP - 1702
EP - 1709
JO - American Journal of Neuroradiology
JF - American Journal of Neuroradiology
IS - 9
ER -