Analysis of a mitotic role of Cdc42

Shingo Yasuda, Shuh Narumiya

    Research output: Contribution to journalReview article

    1 Scopus citations

    Abstract

    Rho GTPases including Rho, Rac, and Cdc42 determine the cell shape by regulating the actin and microtubule dynamics. Through these actions on cytoskeleton, Rho GTPases also regulate cell cycle progression. Specifically, Rho, Rac, and Cdc42 regulate G1-S progression, and Rho regulates cytokinesis. However, involvement of these GTPases in nuclear division has not been definitely shown. This seems to be due to the lack of standard procedures examining mitosis-specific functions of these GTPases. Recently, we have found a mitosis-specific role of Cdc42 by enrichment of cells in mitosis by cell cycle synchronization and interfering with functions of Rho GTPases. This chapter describes the procedures we used in these studies.

    Original languageEnglish (US)
    Article number51
    Pages (from-to)656-665
    Number of pages10
    JournalMethods in enzymology
    Volume406
    DOIs
    StatePublished - Jan 1 2006

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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