Andrographolide mitigates cardiac apoptosis to provide cardio-protection in high-fat-diet-induced obese mice

Kuan Ho Lin, Shibu Marthandam Asokan, Wei Wen Kuo, You Liang Hsieh, Chong Kuei Lii, Vijayapadma Viswanadha, Yi Lin Lin, Shulin Wang, Caixian Yang, Chih Yang Huang

Research output: Contribution to journalArticle

Abstract

Excessive intake of high fat diet (HFD) and associated obese conditions are critical contributors of cardiac diseases. In this study, an active metabolite andrographolide from Andrographis paniculata was found to ameliorate HFD-induced cardiac apoptosis. C57/BL6 mouse were grouped as control (n = 9), obese (n = 8), low dose (25 mg/kg/d) andrographolide treatment (n = 9), and high dose (50 mg/kg/d) andrographolide treatment (n = 9). The control group was provided with standard laboratory chow and the other groups were fed with HFD. Andrographolide was administered through oral gavage for 1 week. Histopathological analysis showed increase in apoptotic nuclei and considerable cardiac-damages in the obese group signifying cardiac remodeling effects. Further, Western blot results showed increase in pro-apoptotic proteins and decrease in the proteins of IGF-1R-survival signaling. However, feeding of andrographolide significantly reduced the cardiac effects of HFD. The results strongly suggest that andrographolide supplementation can be used for prevention and treatment of cardiovascular disease in obese patients.

Original languageEnglish (US)
JournalEnvironmental Toxicology
DOIs
Publication statusAccepted/In press - Jan 1 2020

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Keywords

  • Andrographis paniculata
  • cardiac apoptosis
  • cell survival
  • high fat diet
  • obesity

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

Lin, K. H., Marthandam Asokan, S., Kuo, W. W., Hsieh, Y. L., Lii, C. K., Viswanadha, V., ... Huang, C. Y. (Accepted/In press). Andrographolide mitigates cardiac apoptosis to provide cardio-protection in high-fat-diet-induced obese mice. Environmental Toxicology. https://doi.org/10.1002/tox.22906