Antigen-specific T cell clones restricted by DR, DRw53 (MT), or DP (SB) class II HLA molecules. Inhibition studies with monoclonal HLA-specific antibodies

Erik Qvigstad, Gustav Gaudernack, Erik Thorsby

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

T lymphocyte clones (TLCs) specific for Chlamydia trachomatis were obtained after limiting dilution of activated T cells from a single donor, BS (HLA-A3; B7,15; DR1,4; DRw53; DPw4). Most of the proliferative TLCs obtained apparently used restriction elements on DR1 or DR4 molecules, expressed in the antigen-presenting cells (APC). The restriction pattern of two TLCs, however, closely followed the DRw53 specificity, while one TLC seemed to be restricted by elements on DPw4 molecules. A panel of murine monoclonal antibodies (Mabs) was employed to further dissect the restriction specificities. The proliferative capacity of some of the TLCs was studied with and without Mabs directed against different HLA molecules, using non-T cells, monocytes, and peripheral blood dendritic cells as APC. Two Mabs, 7.2 and D-54, which seem to detect monomorphic determinants on HLA-DR, inhibited both DR- and DRw53-restricted TLCs. One Mab, 109d6 which reacts with DRw53 determinants, selectively inhibited the DRw53-restricted TLCs. The DP-restricted TLC was not inhibited significantly by any tested Mabs, including two Mabs with putative specificity for monomorphic determinants on the DP molecule.

Original languageEnglish (US)
Pages (from-to)207-217
Number of pages11
JournalHuman Immunology
Volume11
Issue number4
DOIs
StatePublished - Jan 1 1984

Fingerprint

Clone Cells
T-Lymphocytes
Antigens
Antibodies
Monoclonal Antibodies
Antigen-Presenting Cells
HLA-A3 Antigen
HLA-B7 Antigen
Chlamydia trachomatis
HLA-DR Antigens
Dendritic Cells
Monocytes
Blood Cells

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Antigen-specific T cell clones restricted by DR, DRw53 (MT), or DP (SB) class II HLA molecules. Inhibition studies with monoclonal HLA-specific antibodies. / Qvigstad, Erik; Gaudernack, Gustav; Thorsby, Erik.

In: Human Immunology, Vol. 11, No. 4, 01.01.1984, p. 207-217.

Research output: Contribution to journalArticle

@article{993f4034083d45a1bbd37eae9be4ebc5,
title = "Antigen-specific T cell clones restricted by DR, DRw53 (MT), or DP (SB) class II HLA molecules. Inhibition studies with monoclonal HLA-specific antibodies",
abstract = "T lymphocyte clones (TLCs) specific for Chlamydia trachomatis were obtained after limiting dilution of activated T cells from a single donor, BS (HLA-A3; B7,15; DR1,4; DRw53; DPw4). Most of the proliferative TLCs obtained apparently used restriction elements on DR1 or DR4 molecules, expressed in the antigen-presenting cells (APC). The restriction pattern of two TLCs, however, closely followed the DRw53 specificity, while one TLC seemed to be restricted by elements on DPw4 molecules. A panel of murine monoclonal antibodies (Mabs) was employed to further dissect the restriction specificities. The proliferative capacity of some of the TLCs was studied with and without Mabs directed against different HLA molecules, using non-T cells, monocytes, and peripheral blood dendritic cells as APC. Two Mabs, 7.2 and D-54, which seem to detect monomorphic determinants on HLA-DR, inhibited both DR- and DRw53-restricted TLCs. One Mab, 109d6 which reacts with DRw53 determinants, selectively inhibited the DRw53-restricted TLCs. The DP-restricted TLC was not inhibited significantly by any tested Mabs, including two Mabs with putative specificity for monomorphic determinants on the DP molecule.",
author = "Erik Qvigstad and Gustav Gaudernack and Erik Thorsby",
year = "1984",
month = "1",
day = "1",
doi = "10.1016/0198-8859(84)90060-0",
language = "English (US)",
volume = "11",
pages = "207--217",
journal = "Human Immunology",
issn = "0198-8859",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Antigen-specific T cell clones restricted by DR, DRw53 (MT), or DP (SB) class II HLA molecules. Inhibition studies with monoclonal HLA-specific antibodies

AU - Qvigstad, Erik

AU - Gaudernack, Gustav

AU - Thorsby, Erik

PY - 1984/1/1

Y1 - 1984/1/1

N2 - T lymphocyte clones (TLCs) specific for Chlamydia trachomatis were obtained after limiting dilution of activated T cells from a single donor, BS (HLA-A3; B7,15; DR1,4; DRw53; DPw4). Most of the proliferative TLCs obtained apparently used restriction elements on DR1 or DR4 molecules, expressed in the antigen-presenting cells (APC). The restriction pattern of two TLCs, however, closely followed the DRw53 specificity, while one TLC seemed to be restricted by elements on DPw4 molecules. A panel of murine monoclonal antibodies (Mabs) was employed to further dissect the restriction specificities. The proliferative capacity of some of the TLCs was studied with and without Mabs directed against different HLA molecules, using non-T cells, monocytes, and peripheral blood dendritic cells as APC. Two Mabs, 7.2 and D-54, which seem to detect monomorphic determinants on HLA-DR, inhibited both DR- and DRw53-restricted TLCs. One Mab, 109d6 which reacts with DRw53 determinants, selectively inhibited the DRw53-restricted TLCs. The DP-restricted TLC was not inhibited significantly by any tested Mabs, including two Mabs with putative specificity for monomorphic determinants on the DP molecule.

AB - T lymphocyte clones (TLCs) specific for Chlamydia trachomatis were obtained after limiting dilution of activated T cells from a single donor, BS (HLA-A3; B7,15; DR1,4; DRw53; DPw4). Most of the proliferative TLCs obtained apparently used restriction elements on DR1 or DR4 molecules, expressed in the antigen-presenting cells (APC). The restriction pattern of two TLCs, however, closely followed the DRw53 specificity, while one TLC seemed to be restricted by elements on DPw4 molecules. A panel of murine monoclonal antibodies (Mabs) was employed to further dissect the restriction specificities. The proliferative capacity of some of the TLCs was studied with and without Mabs directed against different HLA molecules, using non-T cells, monocytes, and peripheral blood dendritic cells as APC. Two Mabs, 7.2 and D-54, which seem to detect monomorphic determinants on HLA-DR, inhibited both DR- and DRw53-restricted TLCs. One Mab, 109d6 which reacts with DRw53 determinants, selectively inhibited the DRw53-restricted TLCs. The DP-restricted TLC was not inhibited significantly by any tested Mabs, including two Mabs with putative specificity for monomorphic determinants on the DP molecule.

UR - http://www.scopus.com/inward/record.url?scp=0021721745&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021721745&partnerID=8YFLogxK

U2 - 10.1016/0198-8859(84)90060-0

DO - 10.1016/0198-8859(84)90060-0

M3 - Article

C2 - 6210280

AN - SCOPUS:0021721745

VL - 11

SP - 207

EP - 217

JO - Human Immunology

JF - Human Immunology

SN - 0198-8859

IS - 4

ER -