Antioxidant activity and protective effects of the Angelica sinensis-hydroxypropyl-β-cyclodextrin complex on CCl4-induced hepatic failure in mice

Chin Mu Hsu, Fuu Jen Tsai, Yuhsin Tsai

Research output: Contribution to journalArticle

Abstract

Angelica sinensis (Oliv.) Diels (AS) is one of the most popular traditional medicines in China. The components of AS extracts have antioxidant activities and protects against hepatic injury, but minimal solubility in water. The use of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) to complex the ethanolic extract of AS has been reported to increase the aqueous solubility of AS components. The aim of this study was to investigate the protective effects of the AS-HP-β-CD complex on CCl4-induced hepatic failure in mice and evaluate the antioxidant properties of this complex. The results showed that the antioxidant activity of the AS-HP-β-CD complex was increased compared with that of the AS extract in a dose-dependent manner. In the CCl4-treated mouse model, the AS-HP-β-CD complex more effectively reduced the increases in serum aspartate aminotransferase, serum alanine transferase, and hepatic malondialdehyde concentrations than the AS extract. Similarly, positive effects were also observed in histopathological specimens and in liver antioxidant enzyme activities. Therefore, the results suggested that complexing AS extract with HP-β-CD improved the hepatoprotective effects of AS.

Original languageEnglish (US)
JournalJournal of Inclusion Phenomena and Macrocyclic Chemistry
DOIs
StateAccepted/In press - Jan 1 2020

Fingerprint

Angelica sinensis
liver failure
cyclodextrins
Liver Failure
Cyclodextrins
antioxidants
protective effect
mice
Antioxidants
antioxidant activity
extracts
serums
water solubility
solubility
Solubility
liver
Liver
aspartates
enzyme activity
alanine

Keywords

  • Angelica sinensis
  • Antioxidant activity
  • Hydroxypropyl-β-cyclodextrin
  • Inclusion complex
  • Liver

ASJC Scopus subject areas

  • Food Science
  • Chemistry(all)
  • Condensed Matter Physics

Cite this

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title = "Antioxidant activity and protective effects of the Angelica sinensis-hydroxypropyl-β-cyclodextrin complex on CCl4-induced hepatic failure in mice",
abstract = "Angelica sinensis (Oliv.) Diels (AS) is one of the most popular traditional medicines in China. The components of AS extracts have antioxidant activities and protects against hepatic injury, but minimal solubility in water. The use of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) to complex the ethanolic extract of AS has been reported to increase the aqueous solubility of AS components. The aim of this study was to investigate the protective effects of the AS-HP-β-CD complex on CCl4-induced hepatic failure in mice and evaluate the antioxidant properties of this complex. The results showed that the antioxidant activity of the AS-HP-β-CD complex was increased compared with that of the AS extract in a dose-dependent manner. In the CCl4-treated mouse model, the AS-HP-β-CD complex more effectively reduced the increases in serum aspartate aminotransferase, serum alanine transferase, and hepatic malondialdehyde concentrations than the AS extract. Similarly, positive effects were also observed in histopathological specimens and in liver antioxidant enzyme activities. Therefore, the results suggested that complexing AS extract with HP-β-CD improved the hepatoprotective effects of AS.",
keywords = "Angelica sinensis, Antioxidant activity, Hydroxypropyl-β-cyclodextrin, Inclusion complex, Liver",
author = "Hsu, {Chin Mu} and Tsai, {Fuu Jen} and Yuhsin Tsai",
year = "2020",
month = "1",
day = "1",
doi = "10.1007/s10847-020-00975-2",
language = "English (US)",
journal = "Journal of Inclusion Phenomena and Macrocyclic Chemistry",
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T1 - Antioxidant activity and protective effects of the Angelica sinensis-hydroxypropyl-β-cyclodextrin complex on CCl4-induced hepatic failure in mice

AU - Hsu, Chin Mu

AU - Tsai, Fuu Jen

AU - Tsai, Yuhsin

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Angelica sinensis (Oliv.) Diels (AS) is one of the most popular traditional medicines in China. The components of AS extracts have antioxidant activities and protects against hepatic injury, but minimal solubility in water. The use of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) to complex the ethanolic extract of AS has been reported to increase the aqueous solubility of AS components. The aim of this study was to investigate the protective effects of the AS-HP-β-CD complex on CCl4-induced hepatic failure in mice and evaluate the antioxidant properties of this complex. The results showed that the antioxidant activity of the AS-HP-β-CD complex was increased compared with that of the AS extract in a dose-dependent manner. In the CCl4-treated mouse model, the AS-HP-β-CD complex more effectively reduced the increases in serum aspartate aminotransferase, serum alanine transferase, and hepatic malondialdehyde concentrations than the AS extract. Similarly, positive effects were also observed in histopathological specimens and in liver antioxidant enzyme activities. Therefore, the results suggested that complexing AS extract with HP-β-CD improved the hepatoprotective effects of AS.

AB - Angelica sinensis (Oliv.) Diels (AS) is one of the most popular traditional medicines in China. The components of AS extracts have antioxidant activities and protects against hepatic injury, but minimal solubility in water. The use of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) to complex the ethanolic extract of AS has been reported to increase the aqueous solubility of AS components. The aim of this study was to investigate the protective effects of the AS-HP-β-CD complex on CCl4-induced hepatic failure in mice and evaluate the antioxidant properties of this complex. The results showed that the antioxidant activity of the AS-HP-β-CD complex was increased compared with that of the AS extract in a dose-dependent manner. In the CCl4-treated mouse model, the AS-HP-β-CD complex more effectively reduced the increases in serum aspartate aminotransferase, serum alanine transferase, and hepatic malondialdehyde concentrations than the AS extract. Similarly, positive effects were also observed in histopathological specimens and in liver antioxidant enzyme activities. Therefore, the results suggested that complexing AS extract with HP-β-CD improved the hepatoprotective effects of AS.

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