Application of NIR fluorescent markers to quantify expression level of HER2 receptors in carcinomas in vivo

Victor Chernomordik, Moinuddin Hassan, Sang Bong Lee, Rafal Zielinski, Jacek Capala, Amir Gandjbakhche

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

HER2 overexpression has been associated with a poor prognosis and resistance to therapy in breast cancer patients. However, quantitative estimates of this important characteristic have been limited to ex vivo ELISA essays of tissue biopsies and/or PET. We develop a novel approach in optical imaging, involving specific probes, not interfering with the binding of the therapeutic agents, thus, excluding competition between therapy and imaging. Affibody-based molecular probes seem to be ideal for in vivo analysis of HER2 receptors using near-infrared optical imaging. Fluorescence intensity distributions, originating from specific markers in the tumor area, can reveal the corresponding fluorophore concentration. We use temporal changes of the signal from a contrast agent, conjugated with HER2-specific Affibody as a signature to monitor in vivo the receptors status in mice with different HER2 over-expressed tumor models. Kinetic model, incorporating saturation of the bound ligands in the tumor area due to HER2 receptor concentration, is suggested to analyze relationship between tumor cell characteristics, i.e., HER2 overexpression, obtained by traditional ("golden standard") ex vivo methods (ELISA), and parameters, estimated from the series of images in vivo. Observed correlation between these parameters and HER2 overexpression substantiates application of our approach to quantify HER2 concentration in vivo.

Original languageEnglish (US)
Title of host publicationOptical Biopsy VII
DOIs
StatePublished - 2010
Externally publishedYes
EventOptical Biopsy VII - San Francisco, CA, United States
Duration: Jan 25 2010Jan 28 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7561
ISSN (Print)1605-7422

Other

OtherOptical Biopsy VII
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/25/101/28/10

Keywords

  • Clinical applications
  • HER2 overexpression
  • Medical and biological imaging
  • Spectroscopy and fluorescence

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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