APS, an adapter protein with a PH and SH2 domain, is a substrate for the insulin receptor kinase

Zamal Ahmed, Beverley J. Smith, Kei Kotani, Peter Wilden, Tahir S. Pillay

Research output: Contribution to journalArticlepeer-review

53 Scopus citations

Abstract

APS (adapter protein with a PH and SH2 domain) is the newest member of a family of tyrosine kinase adapter proteins including SH2-B and Lnk. We previously identified SH2-B as an insulin-receptor-binding protein and substrate. Here we show that APS interacts with the insulin receptor kinase activation loop through its SH2 domain and insulin stimulates the tyrosine-phosphorylation of APS. Furthermore, the phosphorylation of activation-loop tyrosine residues 1158 and 1162 are required for this interaction.

Original languageEnglish (US)
Pages (from-to)665-668
Number of pages4
JournalBiochemical Journal
Volume341
Issue number3
DOIs
StatePublished - Aug 1 1999
Externally publishedYes

Keywords

  • Tyrosine kinase
  • Tyrosine phosphorylation
  • Yeast two-hybrid system

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'APS, an adapter protein with a PH and SH2 domain, is a substrate for the insulin receptor kinase'. Together they form a unique fingerprint.

Cite this