Abstract
APS (adapter protein with a PH and SH2 domain) is the newest member of a family of tyrosine kinase adapter proteins including SH2-B and Lnk. We previously identified SH2-B as an insulin-receptor-binding protein and substrate. Here we show that APS interacts with the insulin receptor kinase activation loop through its SH2 domain and insulin stimulates the tyrosine-phosphorylation of APS. Furthermore, the phosphorylation of activation-loop tyrosine residues 1158 and 1162 are required for this interaction.
Original language | English (US) |
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Pages (from-to) | 665-668 |
Number of pages | 4 |
Journal | Biochemical Journal |
Volume | 341 |
Issue number | 3 |
DOIs | |
State | Published - Aug 1 1999 |
Externally published | Yes |
Keywords
- Tyrosine kinase
- Tyrosine phosphorylation
- Yeast two-hybrid system
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology