Arvanil and anandamide up-regulate CD36 expression in human peripheral blood mononuclear cells

Anna Maria Malfitano, Gokce Altay Toruner, Patrizia Gazzerro, Chiara Laezza, Seema Husain, Davide Eletto, Pierangelo Orlando, Luciano De Petrocellis, Alexandra Terskiy, Marvin Schwalb, Emilia Vitale, Maurizio Bifulco

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In this study we analysed the regulation of gene expression by arvanil and anandamide in human peripheral blood mononuclear cells (PBMCs) to clarify their immunosuppressive properties. PBMCs were activated, leading to CD36 down regulation, that was normalized by arvanil and anandamide. We used microarray technology to identify a regulatory pattern associated with cell proliferation in the presence of both substances. CD3-CD28 stimulated PBMCs showed a pattern of up-regulated and down-regulated genes after treatment with these substances. We selected and analysed several genes chosen by their function in the regulation of cell proliferation. We showed a transcriptional control of the CD36 gene by arvanil and anandamide associated with an increased protein expression, thus suggesting a possible role of CD36 in anandamide and arvanil anti-inflammatory pattern.

Original languageEnglish (US)
Pages (from-to)145-154
Number of pages10
JournalImmunology Letters
Volume109
Issue number2
DOIs
StatePublished - Apr 15 2007
Externally publishedYes

Keywords

  • Anadamide
  • Arvanil
  • CD36
  • Human peripheral blood mononuclear cells (PBMCs)
  • RNA microarray

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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