TY - JOUR
T1 - Assessment of Ablative Therapies in Swine
T2 - Response of Respiratory Diaphragm to Varying Doses
AU - Singal, Ashish
AU - Mattison, Lars M.
AU - Soule, Charles L.
AU - Ballard, John R.
AU - Rudie, Eric N.
AU - Cressman, Erik N.K.
AU - Iaizzo, Paul A.
N1 - Publisher Copyright:
© 2018, Biomedical Engineering Society.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Ablation is a common procedure for treating patients with cancer, cardiac arrhythmia, and other conditions, yet it can cause collateral injury to the respiratory diaphragm. Collateral injury can alter the diaphragm’s properties and/or lead to respiratory dysfunction. Thus, it is important to understand the diaphragm’s physiologic and biomechanical properties in response to ablation therapies, in order to better understand ablative modalities, minimize complications, and maximize the safety and efficacy of ablative procedures. In this study, we analyzed physiologic and biomechanical properties of swine respiratory diaphragm muscle bundles when exposed to 5 ablative modalities. To assess physiologic properties, we performed in vitro tissue bath studies and measured changes in peak force and baseline force. To assess biomechanical properties, we performed uniaxial stress tests, measuring force–displacement responses, stress–strain characteristics, and avulsion forces. After treating the muscle bundles with all 5 ablative modalities, we observed dose-dependent sustained reductions in peak force and transient increases in baseline force—but no consistent dose-dependent biomechanical responses. These data provide novel insights into the effects of various ablative modalities on the respiratory diaphragm, insights that could enable improvements in ablative techniques and therapies.
AB - Ablation is a common procedure for treating patients with cancer, cardiac arrhythmia, and other conditions, yet it can cause collateral injury to the respiratory diaphragm. Collateral injury can alter the diaphragm’s properties and/or lead to respiratory dysfunction. Thus, it is important to understand the diaphragm’s physiologic and biomechanical properties in response to ablation therapies, in order to better understand ablative modalities, minimize complications, and maximize the safety and efficacy of ablative procedures. In this study, we analyzed physiologic and biomechanical properties of swine respiratory diaphragm muscle bundles when exposed to 5 ablative modalities. To assess physiologic properties, we performed in vitro tissue bath studies and measured changes in peak force and baseline force. To assess biomechanical properties, we performed uniaxial stress tests, measuring force–displacement responses, stress–strain characteristics, and avulsion forces. After treating the muscle bundles with all 5 ablative modalities, we observed dose-dependent sustained reductions in peak force and transient increases in baseline force—but no consistent dose-dependent biomechanical responses. These data provide novel insights into the effects of various ablative modalities on the respiratory diaphragm, insights that could enable improvements in ablative techniques and therapies.
KW - Chemical ablation
KW - Cryoablation
KW - High-intensity focused ultrasound ablation
KW - Microwave ablation
KW - Radiofrequency ablation
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U2 - 10.1007/s10439-018-2014-x
DO - 10.1007/s10439-018-2014-x
M3 - Article
C2 - 29594687
AN - SCOPUS:85044439838
SN - 0090-6964
VL - 46
SP - 947
EP - 959
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - 7
ER -