Assessment of In Vivo siRNA Delivery in Cancer Mouse Models

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

RNA interference (RNAi) has rapidly become a powerful tool for target discovery and therapeutics. Small interfering RNAs (siRNAs) are highly effective in mediating sequence-specific gene silencing. However, the major obstacle for using siRNAs for cancer therapeutics is their systemic delivery from the administration site to target cells in vivo. This chapter describes approaches to deliver siRNA effectively for cancer treatment and discusses in detail the current methods to assess pharmacokinetics and biodistribution of siRNAs in vivo.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages157-168
Number of pages12
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2372
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cancer Therapy
  • Chitosan-siRNA Nanoparticles
  • Ovarian Cancer
  • Stem-loop RT-PCR
  • siRNA Delivery

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

MD Anderson CCSG core facilities

  • Small Animal Imaging Facility

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