@inbook{959f1735463b4922878f41b3f11ad9b8,
title = "Assessment of intracellular Mucin content in vivo",
abstract = "Airway mucus presents a first line of defense against inhaled materials. It also, however, is a significant pathological contributor to chronic lung diseases, such as asthma, cystic fibrosis, and chronic obstructive pulmonary disease. Thus, gaining a better understanding of the mechanisms of mucus production and secretion is an important goal for improving respiratory health. Mucins, the chief glycoprotein components of airway mucus, are very large polymeric glycoproteins, and measuring their production and secretion in experimental animals presents significant technical challenges. Over the past several years, we have developed assays for accurately quantifying mucin production and secretion using histological and biochemical assays. These methods are described here.",
keywords = "Airways, Asthma, Chronic obstructive pulmonary disease, Cystic fibrosis, Goblet cell, Lungs, Mouse, Mucin, Mucous, Mucus",
author = "Lucia Piccotti and Dickey, {Burton F.} and Evans, {Christopher M.}",
year = "2012",
doi = "10.1007/978-1-61779-513-8_17",
language = "English (US)",
isbn = "9781617795121",
series = "Methods in Molecular Biology",
pages = "279--295",
editor = "Michael McGuckin and David Thornton",
booktitle = "Mucins",
}