@inbook{393c009877e04fe79cd7f6fa87479bd7,
title = "Assessment of lipid peroxidation in irradiated cells",
abstract = "Lipid peroxidation occurs under conditions where reactive oxygen species (ROS) readily react with vulnerable lipids on cell membranes. Polyunsaturated fatty acids (PUFAs) are highly susceptible to lipid peroxidation because of their unstable double bonds. Because the cell membrane is particularly rich in PUFAs, it is often the site at which many lipid peroxidation chain reactions occur. Lipid peroxidation is considered the ultimate trigger of ferroptosis, an iron-dependent form of non-apoptotic cell death. Radiotherapy is a common cancer treatment that uses high-energy ionizing radiation to kill cancer cells, and radiation-induced cell death is partially attributed to lipid peroxidation-driven ferroptosis. Here, we describe methods to assess lipid peroxidation in irradiated cells. The same techniques can be applied to a variety of lipid peroxidation measurements under different treatment conditions.",
keywords = "Cell death, Ferroptosis, Lipid peroxidation, Radiation, Reactive oxygen species",
author = "Chao Mao and Guang Lei and Amber Horbath and Boyi Gan",
note = "Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = jan,
doi = "10.1016/bs.mcb.2022.05.003",
language = "English (US)",
isbn = "9780323899499",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "37--50",
editor = "Ai Sato and Jeffrey Kraynak and Marciscano, {Ariel E.} and Lorenzo Galluzzi",
booktitle = "Radiation Oncology and Radiotherapy Part A",
}