@inbook{ffb570942b6341398f0dc0ca7b8c8215,
title = "Assessment of the Murine Tumor Microenvironment by Multiplex Immunofluorescence",
abstract = "We describe the staining methods used for simultaneous detection of tumor microenvironment components as well as the automated quantification methodologies. This method uses mouse formalin-fixed paraffin-embedded tissues and multiplex immunofluorescence (Multiplex IF) followed by multispectral imaging. Currently, this methodology has shown to have a valuable role in murine immunoprofiling, and can be useful when evaluating the changes incurred on the tumor microenvironment upon various immunopreventive strategies.",
keywords = "Immune markers, Multiplex IF, Opal{\texttrademark}, Quantification algorithms, Vectra",
author = "Quesada, {Pompeyo R.} and Erick Riquelme and Jared Burks and Amanda Rakoski and Ismet Sahin and Florencia McAllister",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
doi = "10.1007/978-1-0716-2014-4_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "107--127",
booktitle = "Methods in Molecular Biology",
}