TY - JOUR
T1 - Atomic force and total internal reflection fluorescence microscopy for the study of force transmission in endothelial cells
AU - Mathur, Anshu
AU - Reichert, William
AU - Truskey, George
N1 - Funding Information:
This work was supported by National Institute of Health grants HL32132 and HL44972. We also acknowledge the Comprehensive Cancer Center shared Confocal Microscope facility.
PY - 2000
Y1 - 2000
N2 - Atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) were configured to examine the transmission of force applied from the apical cell membrane to the basal cell membrane of HUVECs. The precise localized mechanical perturbations, resulted in a global rearrangement of focal contacts at the basal membrane. The apical cell surface displayed a range of elastic moduli. Although the response remained global whether the region was soft or stiff, force application over the nuclear region appeared to produce a more consistent change in contact area, while the contact area responded variably to the force on the edge of the cell.
AB - Atomic force microscopy (AFM) and total internal reflection fluorescence microscopy (TIRFM) were configured to examine the transmission of force applied from the apical cell membrane to the basal cell membrane of HUVECs. The precise localized mechanical perturbations, resulted in a global rearrangement of focal contacts at the basal membrane. The apical cell surface displayed a range of elastic moduli. Although the response remained global whether the region was soft or stiff, force application over the nuclear region appeared to produce a more consistent change in contact area, while the contact area responded variably to the force on the edge of the cell.
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M3 - Conference article
AN - SCOPUS:0034516153
SN - 0090-6964
VL - 28
SP - S-81
JO - Annals of Biomedical Engineering
JF - Annals of Biomedical Engineering
IS - SUPPL. 1
T2 - 2000 Annual Fall Meeting of the Biomedical Engineering Society
Y2 - 12 October 2000 through 14 October 2000
ER -