Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring

Adetunji P. Fayomi; Karen Peters; Meena Sukhwani; Hanna Valli-Pulaski; Gunapala Shetty; Marvin L. Meistrich; Lisa Houser; Nicola Robertson; Victoria Roberts; Cathy Ramsey; Carol Hanna; Jon D. Hennebold; Ina Dobrinski; Kyle E. Orwig

doi:10.1126/science.aav2914

Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring

Adetunji P. Fayomi, Karen Peters, Meena Sukhwani, Hanna Valli-Pulaski, Gunapala Shetty, Marvin L. Meistrich, Lisa Houser, Nicola Robertson, Victoria Roberts, Cathy Ramsey, Carol Hanna, Jon D. Hennebold, Ina Dobrinski, Kyle E. Orwig

Experimental Radiation Oncology

Research output: Contribution to journal › Article › peer-review

199 Scopus citations

Abstract

Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.

Original language	English (US)
Pages (from-to)	1314-1319
Number of pages	6
Journal	Science
Volume	363
Issue number	6433
DOIs	https://doi.org/10.1126/science.aav2914
State	Published - Mar 22 2019

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Fayomi, A. P., Peters, K., Sukhwani, M., Valli-Pulaski, H., Shetty, G., Meistrich, M. L., Houser, L., Robertson, N., Roberts, V., Ramsey, C., Hanna, C., Hennebold, J. D., Dobrinski, I., & Orwig, K. E. (2019). Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring. Science, 363(6433), 1314-1319. https://doi.org/10.1126/science.aav2914

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abstract = "Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.",

author = "Fayomi, {Adetunji P.} and Karen Peters and Meena Sukhwani and Hanna Valli-Pulaski and Gunapala Shetty and Meistrich, {Marvin L.} and Lisa Houser and Nicola Robertson and Victoria Roberts and Cathy Ramsey and Carol Hanna and Hennebold, {Jon D.} and Ina Dobrinski and Orwig, {Kyle E.}",

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AU - Fayomi, Adetunji P.

AU - Peters, Karen

AU - Sukhwani, Meena

AU - Valli-Pulaski, Hanna

AU - Shetty, Gunapala

AU - Meistrich, Marvin L.

AU - Houser, Lisa

AU - Robertson, Nicola

AU - Roberts, Victoria

AU - Ramsey, Cathy

AU - Hanna, Carol

AU - Hennebold, Jon D.

AU - Dobrinski, Ina

AU - Orwig, Kyle E.

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AB - Testicular tissue cryopreservation is an experimental method to preserve the fertility of prepubertal patients before they initiate gonadotoxic therapies for cancer or other conditions. Here we provide the proof of principle that cryopreserved prepubertal testicular tissues can be autologously grafted under the back skin or scrotal skin of castrated pubertal rhesus macaques and matured to produce functional sperm. During the 8- to 12-month observation period, grafts grew and produced testosterone. Complete spermatogenesis was confirmed in all grafts at the time of recovery. Graft-derived sperm were competent to fertilize rhesus oocytes, leading to preimplantation embryo development, pregnancy, and the birth of a healthy female baby. Pending the demonstration that similar results are obtained in noncastrated recipients, testicular tissue grafting may be applied in the clinic.

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Autologous grafting of cryopreserved prepubertal rhesus testis produces sperm and offspring

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