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Automated Lymph Node and Extranodal Extension Assessment Improves Risk Stratification in Oropharyngeal Carcinoma

  • Zezhong Ye
  • , Reza Mojahed-Yazdi
  • , Anna Zapaishchykova
  • , Divyanshu Tak
  • , Maryam Mahootiha
  • , Juan Carlos Climent Pardo
  • , John Zielke
  • , Yining Zha
  • , Christian Guthier
  • , Roy B. Tishler
  • , Danielle N. Margalit
  • , Jonathan D. Schoenfeld
  • , Robert I. Haddad
  • , Ravindra Uppaluri
  • , Benjamin Haibe-Kains
  • , Clifton D. Fuller
  • , Mohamed Naser
  • , Barbara A. Burtness
  • , Hugo J.W.L. Aerts
  • , Frank Hoebers
  • Benjamin H. Kann

Research output: Contribution to journalArticlepeer-review

Abstract

PURPOSE – Extranodal extension (ENE) is a biomarker in oropharyngeal carcinoma (OPC) but can only be diagnosed via surgical pathology. We applied an automated artificial intelligence (AI) imaging platform integrating lymph node autosegmentation with ENE prediction to determine the prognostic value of the number of predicted ENE nodes.MATERIALS AND METHODS – We conducted a multisite, retrospective study of 1, 733 OPC patients with pretreatment computed tomography who underwent definitive radiation therapy across three institutions. Malignant lymph nodes were segmented using a validated deep learning auto-segmentation model, and segmented lymph nodes were sequentially processed with a validated ENE prediction model to calculate number of nodes with AI-predicted ENE (AI-ENE) per patient. We evaluated associations of AI-ENE with disease outcomes using site-stratified, multivariable Cox regression, adjusting for human papillomavirus (HPV) status, smoking pack-years, tumor and nodal stage, age, and sex. We evaluated risk-stratification improvement when incorporating AI-ENE into the Radiation Therapy Oncology Group (RTOG)-0129 risk groupings and derived American Joint Committee on Cancer (AJCC) 8th edition staging with Uno C-indices and decision curve analyses.RESULTS – Overall, median AI-ENE node number was 1 (range, 0-6). AI-ENE node number was independently associated with poorer distant control (DC; hazard ratio [HR], 1.44 [95% CI, 1.23 to 1.69]; P < .001) and overall survival (OS; HR, 1.30 [95% CI, 1.16 to 1.46]; P < .001). Increasing AI-ENE node number was incrementally associated with worse outcome, particularly DC (P < .001). C-indices improved in the external data set when incorporating AI-ENE into RTOG-0129 groupings (OS: 0.70 v 0.65; DC: 0.65 v 0.57) and AJCC-8 stage (OS: 0.75 v 0.70; DC: 0.72 v 0.67; P < .001 for each). The largest improvements were observed among HPV-negative patients (C-index: +15% for OS, +14% for DC).CONCLUSION – Automated, AI-ENE node number is a novel risk factor for OPC that may better inform pretreatment risk stratification and decision-making.

Original languageEnglish (US)
Pages (from-to)386-399
Number of pages14
JournalJournal of Clinical Oncology
Volume44
Issue number5
DOIs
StatePublished - Feb 10 2026

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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