Baicalein induces apoptosis in SCC-4 human tongue cancer cells via a Ca2+-dependent mitochondrial pathway

Yuh Tzy Lin, Jai Sing Yang, Hui Ju Lin, Tzu Wei Tan, Nou Ying Tang, Jo Hua Chaing, Yung Hsien Chang, Hsu Feng Lu, Jing Gung Chung

Research output: Contribution to journalArticle

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Abstract

Background: The effects of baicalein on SCC-4 human tongue cancer cells were examined to better understand its effect on apoptosis and associated possible signal pathways in vitro. Materials and Methods: Apoptosis induction, reactive oxygen species (ROS), cytoplasmic Ca2+, mitochondrial membrane potential (MMP) and caspase-3 activity were analyzed using the flow cytometric assay. Apoptosis-associated proteins, such as p53, BAX, BCL-2, cytochrome c, caspase-3 and -9, EndoG and AIF were determined by Western blotting. Results: Our results showed that baicalein promoted the levels of p53, BAX, cytochrome c, capase-3 and -9 and reduced the level of BCL-2, which were associated with the induction of apoptotic cell death of SCC-4 cells. A release of cytochrome c from mitochondria into cytosol was demonstrated and an activation of caspase-3, which led to the occurrence of apoptosis in SCC-4 cells treated with baicalein as determined by Western blot. In order to understand the role of Ca2+ in the induction of apoptosis, cells were pre-treated with BAPTA (intracellular calcium chelator) and baicalein. It was shown that the MMP was restored, and the level of cytoplasmic Ca2+ suppressed, the proportion of cells undergoing apoptosis was also markedly diminished. Our data suggest that cellular Ca2+ modulates baicalein-induced cell death via a Ca2+-dependent mitochondrial death pathway in SCC-4 cells.

Original languageEnglish (US)
Pages (from-to)1053-1058
Number of pages6
JournalIn Vivo
Volume21
Issue number6
StatePublished - Nov 1 2007

Fingerprint

Tongue Neoplasms
Cells
Apoptosis
Cytochromes c
Caspase 3
Mitochondrial Membrane Potential
Cell death
Cell Death
Western Blotting
Membranes
Mitochondria
Caspase 9
Cytosol
baicalein
Assays
Reactive Oxygen Species
Signal Transduction
Chemical activation

Keywords

  • Apoptosis
  • Baicalein
  • Caspase-3
  • Cytochrome c
  • Cytoplasmic Ca
  • Mitochondrial death pathway
  • Reactive oxygen species
  • SCC-4 cells

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Lin, Y. T., Yang, J. S., Lin, H. J., Tan, T. W., Tang, N. Y., Chaing, J. H., ... Chung, J. G. (2007). Baicalein induces apoptosis in SCC-4 human tongue cancer cells via a Ca2+-dependent mitochondrial pathway. In Vivo, 21(6), 1053-1058.

Baicalein induces apoptosis in SCC-4 human tongue cancer cells via a Ca2+-dependent mitochondrial pathway. / Lin, Yuh Tzy; Yang, Jai Sing; Lin, Hui Ju; Tan, Tzu Wei; Tang, Nou Ying; Chaing, Jo Hua; Chang, Yung Hsien; Lu, Hsu Feng; Chung, Jing Gung.

In: In Vivo, Vol. 21, No. 6, 01.11.2007, p. 1053-1058.

Research output: Contribution to journalArticle

Lin, YT, Yang, JS, Lin, HJ, Tan, TW, Tang, NY, Chaing, JH, Chang, YH, Lu, HF & Chung, JG 2007, 'Baicalein induces apoptosis in SCC-4 human tongue cancer cells via a Ca2+-dependent mitochondrial pathway', In Vivo, vol. 21, no. 6, pp. 1053-1058.
Lin YT, Yang JS, Lin HJ, Tan TW, Tang NY, Chaing JH et al. Baicalein induces apoptosis in SCC-4 human tongue cancer cells via a Ca2+-dependent mitochondrial pathway. In Vivo. 2007 Nov 1;21(6):1053-1058.
Lin, Yuh Tzy ; Yang, Jai Sing ; Lin, Hui Ju ; Tan, Tzu Wei ; Tang, Nou Ying ; Chaing, Jo Hua ; Chang, Yung Hsien ; Lu, Hsu Feng ; Chung, Jing Gung. / Baicalein induces apoptosis in SCC-4 human tongue cancer cells via a Ca2+-dependent mitochondrial pathway. In: In Vivo. 2007 ; Vol. 21, No. 6. pp. 1053-1058.
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AU - Lin, Yuh Tzy

AU - Yang, Jai Sing

AU - Lin, Hui Ju

AU - Tan, Tzu Wei

AU - Tang, Nou Ying

AU - Chaing, Jo Hua

AU - Chang, Yung Hsien

AU - Lu, Hsu Feng

AU - Chung, Jing Gung

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N2 - Background: The effects of baicalein on SCC-4 human tongue cancer cells were examined to better understand its effect on apoptosis and associated possible signal pathways in vitro. Materials and Methods: Apoptosis induction, reactive oxygen species (ROS), cytoplasmic Ca2+, mitochondrial membrane potential (MMP) and caspase-3 activity were analyzed using the flow cytometric assay. Apoptosis-associated proteins, such as p53, BAX, BCL-2, cytochrome c, caspase-3 and -9, EndoG and AIF were determined by Western blotting. Results: Our results showed that baicalein promoted the levels of p53, BAX, cytochrome c, capase-3 and -9 and reduced the level of BCL-2, which were associated with the induction of apoptotic cell death of SCC-4 cells. A release of cytochrome c from mitochondria into cytosol was demonstrated and an activation of caspase-3, which led to the occurrence of apoptosis in SCC-4 cells treated with baicalein as determined by Western blot. In order to understand the role of Ca2+ in the induction of apoptosis, cells were pre-treated with BAPTA (intracellular calcium chelator) and baicalein. It was shown that the MMP was restored, and the level of cytoplasmic Ca2+ suppressed, the proportion of cells undergoing apoptosis was also markedly diminished. Our data suggest that cellular Ca2+ modulates baicalein-induced cell death via a Ca2+-dependent mitochondrial death pathway in SCC-4 cells.

AB - Background: The effects of baicalein on SCC-4 human tongue cancer cells were examined to better understand its effect on apoptosis and associated possible signal pathways in vitro. Materials and Methods: Apoptosis induction, reactive oxygen species (ROS), cytoplasmic Ca2+, mitochondrial membrane potential (MMP) and caspase-3 activity were analyzed using the flow cytometric assay. Apoptosis-associated proteins, such as p53, BAX, BCL-2, cytochrome c, caspase-3 and -9, EndoG and AIF were determined by Western blotting. Results: Our results showed that baicalein promoted the levels of p53, BAX, cytochrome c, capase-3 and -9 and reduced the level of BCL-2, which were associated with the induction of apoptotic cell death of SCC-4 cells. A release of cytochrome c from mitochondria into cytosol was demonstrated and an activation of caspase-3, which led to the occurrence of apoptosis in SCC-4 cells treated with baicalein as determined by Western blot. In order to understand the role of Ca2+ in the induction of apoptosis, cells were pre-treated with BAPTA (intracellular calcium chelator) and baicalein. It was shown that the MMP was restored, and the level of cytoplasmic Ca2+ suppressed, the proportion of cells undergoing apoptosis was also markedly diminished. Our data suggest that cellular Ca2+ modulates baicalein-induced cell death via a Ca2+-dependent mitochondrial death pathway in SCC-4 cells.

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KW - Baicalein

KW - Caspase-3

KW - Cytochrome c

KW - Cytoplasmic Ca

KW - Mitochondrial death pathway

KW - Reactive oxygen species

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