TY - JOUR
T1 - Baseline oral microbiome and all-cancer incidence in a cohort of nonsmoking Mexican American women
AU - Zhang, Xiaotao
AU - Hoffman, Kristi L.
AU - Wei, Peng
AU - Elhor Gbito, Kplola Y.
AU - Joseph, Reji
AU - Li, Fangyu
AU - Scheet, Paul
AU - Chang, Shine
AU - Petrosino, Joseph F.
AU - Daniel, Carrie R.
N1 - Funding Information:
We wish to thank the gracious participants of this study for their continued contributions to preventing and understanding cancer within their community. We wish to acknowledge the cohort leadership, mentorship, and contributions of Dr. Margaret R. Spitz, Dr. Wong Ho Chow (retired), and Ms. Qiong Dong (retired). Cancer data have been provided by the Texas Cancer Registry, Cancer Epidemiology and Surveillance Branch, Texas Department of State Health Services (Austin, TX; https://www.dshs.texas.gov/tcr/). This work was funded (in part) by a Research Training Award for Cancer Prevention Post-Graduate Training Program in Integrative Epidemiology from the Cancer Prevention & Research Institute of Texas RP160097 (to principal investigator, M. Spitz supporting X. Zhang); through the NCI Cancer Center Support Grant (5P30 CA016672–37 to MD Anderson, principal investigator, P. Pisters supporting C.R. Daniel, P. Scheet, S. Chang, and P. Wei); the NCI Cancer Research Training Program (R25CA057730 to principal investigator, S. Chang supporting K.L. Hoffman); and through a Susan G. Komen training grant to reduce breast cancer disparities in Black and Hispanic women (GTDR17498270 to principal investigators, L. McNeill and K. Hunt supporting F. Li). The cohort additionally received funds from the Comprehensive Tobacco Settlement of 1998, Caroline W Law Fund, and Dan Duncan Family Institute for Risk Assessment and Cancer Prevention.
Publisher Copyright:
© 2020 American Association for Cancer Research.
PY - 2021/3
Y1 - 2021/3
N2 - Given the increasing evidence that the oral microbiome is involved in obesity, diabetes, and cancer risk, we investigated baseline oral microbiota profiles in relation to all-cancer incidence among nonsmoking women enrolled in a Texas cohort of first- and second-generation immigrants of Mexican origin. We characterized the 16Sv4 rDNA microbiome in oral mouthwash samples collected at baseline from a representative subset of 305 nonsmoking women, ages 20–75 years. We evaluated within- (alpha) and between-sample (beta) diversity by incident cancer status and applied linear discriminant analysis (LDA) effect size analysis to assess differentially abundant taxa. Diversity and candidate taxa in relation to all-cancer incidence were evaluated in multivariable-adjusted Cox regression models. Over 8.8 median years of follow-up, 31 incident cancer cases were identified and verified. Advanced age, greater acculturation, and cardiometabolic risk factors were associated with all-cancer incidence. Higher alpha diversity (age-adjusted Pdifference < 0.01) and distinct biological communities (Pdifference = 0.002) were observed by incident cancer status. Each unit increase in the Shannon diversity index yielded >8-fold increase in all-cancer and obesity-related cancer risk [multivariable-adjusted HR (95% confidence interval), 8.11 (3.14–20.94) and 10.72 (3.30–34.84), respectively] with similar findings for the inverse Simpson index. Streptococcus was enriched among women who did not develop cancer, while Fusobacterium, Prevotella, Mogibacterium, Campylobacter, Lachnoanaerobaculum, Dialister, and Atopobium were higher among women who developed cancer (LDA score ≥ 3; q-value < 0.01). This initial study of oral microbiota and overall cancer risk in nonsmoking Mexican American women suggests the readily accessible oral microbiota as a promising biomarker.
AB - Given the increasing evidence that the oral microbiome is involved in obesity, diabetes, and cancer risk, we investigated baseline oral microbiota profiles in relation to all-cancer incidence among nonsmoking women enrolled in a Texas cohort of first- and second-generation immigrants of Mexican origin. We characterized the 16Sv4 rDNA microbiome in oral mouthwash samples collected at baseline from a representative subset of 305 nonsmoking women, ages 20–75 years. We evaluated within- (alpha) and between-sample (beta) diversity by incident cancer status and applied linear discriminant analysis (LDA) effect size analysis to assess differentially abundant taxa. Diversity and candidate taxa in relation to all-cancer incidence were evaluated in multivariable-adjusted Cox regression models. Over 8.8 median years of follow-up, 31 incident cancer cases were identified and verified. Advanced age, greater acculturation, and cardiometabolic risk factors were associated with all-cancer incidence. Higher alpha diversity (age-adjusted Pdifference < 0.01) and distinct biological communities (Pdifference = 0.002) were observed by incident cancer status. Each unit increase in the Shannon diversity index yielded >8-fold increase in all-cancer and obesity-related cancer risk [multivariable-adjusted HR (95% confidence interval), 8.11 (3.14–20.94) and 10.72 (3.30–34.84), respectively] with similar findings for the inverse Simpson index. Streptococcus was enriched among women who did not develop cancer, while Fusobacterium, Prevotella, Mogibacterium, Campylobacter, Lachnoanaerobaculum, Dialister, and Atopobium were higher among women who developed cancer (LDA score ≥ 3; q-value < 0.01). This initial study of oral microbiota and overall cancer risk in nonsmoking Mexican American women suggests the readily accessible oral microbiota as a promising biomarker.
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U2 - 10.1158/1940-6207.CAPR-20-0405
DO - 10.1158/1940-6207.CAPR-20-0405
M3 - Article
C2 - 33277317
AN - SCOPUS:85102110188
SN - 1940-6207
VL - 14
SP - 383
EP - 392
JO - Cancer Prevention Research
JF - Cancer Prevention Research
IS - 3
ER -