Biological activity of recombinant human interleukin-2 produced in Escherichia coli

Steven A. Rosenberg, Elizabeth A Grimm, Michael Mcgrogan, Michael Doyle, Ernest Kawasaki, Kirston Koths, David F. Mark

Research output: Contribution to journalArticle

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Abstract

The gene for interleukin-2 was isolated from the Jurkat cell line and from normal peripheral blood lymphocytes and, when inserted in Escherichia coli, was expressed at high concentrations. This interleukin-2 was purified to apparent homogeneity and tested for biological activity in a variety of assays in vitro and in vivo. The recombinant lymphokine supports the growth of murine and human interleukin-2 dependent cell lines, enhances the generation of murine and human cytolytic cells in vitro, and generates lymphokine activated killer cells from murine and human lymphocytes. It has a serum half-life of 2 to 3 minutes in the mouse and significantly enhances the generation of cytolytic cells in vivo after alloimmunization, No functional differences between native and the recombinant interleukin-2 molecules have been detected.

Original languageEnglish (US)
Pages (from-to)1412-1415
Number of pages4
JournalScience
Volume223
Issue number4643
StatePublished - Jan 1 1984

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Human Activities
Interleukin-2
Escherichia coli
Lymphocytes
Lymphokine-Activated Killer Cells
Cell Line
Jurkat Cells
Lymphokines
Half-Life
Growth
Serum
Genes
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Rosenberg, S. A., Grimm, E. A., Mcgrogan, M., Doyle, M., Kawasaki, E., Koths, K., & Mark, D. F. (1984). Biological activity of recombinant human interleukin-2 produced in Escherichia coli. Science, 223(4643), 1412-1415.

Biological activity of recombinant human interleukin-2 produced in Escherichia coli. / Rosenberg, Steven A.; Grimm, Elizabeth A; Mcgrogan, Michael; Doyle, Michael; Kawasaki, Ernest; Koths, Kirston; Mark, David F.

In: Science, Vol. 223, No. 4643, 01.01.1984, p. 1412-1415.

Research output: Contribution to journalArticle

Rosenberg, SA, Grimm, EA, Mcgrogan, M, Doyle, M, Kawasaki, E, Koths, K & Mark, DF 1984, 'Biological activity of recombinant human interleukin-2 produced in Escherichia coli', Science, vol. 223, no. 4643, pp. 1412-1415.
Rosenberg SA, Grimm EA, Mcgrogan M, Doyle M, Kawasaki E, Koths K et al. Biological activity of recombinant human interleukin-2 produced in Escherichia coli. Science. 1984 Jan 1;223(4643):1412-1415.
Rosenberg, Steven A. ; Grimm, Elizabeth A ; Mcgrogan, Michael ; Doyle, Michael ; Kawasaki, Ernest ; Koths, Kirston ; Mark, David F. / Biological activity of recombinant human interleukin-2 produced in Escherichia coli. In: Science. 1984 ; Vol. 223, No. 4643. pp. 1412-1415.
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