Brain perivascular macrophages do not mediate interleukin-1-induced sickness behavior in rats

Léa Chaskiel, Robert Dantzer, Jan Pieter Konsman

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Sickness behavior, characterized by on overall reduction in behavioral activity, is commonly observed after bacterial infection. Sickness behavior can also be induced by the peripheral administration of Gram-negative bacterial lipopolysaccharide (LPS) or interleukin-1beta (IL-1β), a pro-inflammatory cytokine released by LPS-activated macrophages. In addition to the microglia, the brain contains perivascular macrophages, which express the IL-1 type 1 receptor (IL-1R1). In the present study, we assessed the role of brain perivascular macrophages in mediating IL-1β-induced sickness behavior in rats. To do so, we used intracerebroventricular (icv) administration of an IL-1β-saporin conjugate, known to eliminate IL-R1-expressing brain cells, prior to systemic or central IL-1β injection. Icv IL-1β-saporin administration resulted in a reduction in brain perivascular mac-rophages, without altering subsequent icv or ip IL-1β-induced reductions in food intake, locomotor activity, and social interactions. In conclusion, the present work shows that icv IL-1β-saporin administration is an efficient way to target brain perivascular macrophages, and to determine whether these cells are involved in IL-1β-induced sickness behavior.

Original languageEnglish (US)
Article number1030
JournalPharmaceuticals
Volume14
Issue number10
DOIs
StatePublished - Oct 2021

Keywords

  • Brain perivascular macrophages
  • Food intake
  • Interleukin-1
  • Locomotor activity
  • Social interaction

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery

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