Breeding Strategies for Genetically Modified Mice

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Genetically modified mice can be generated using a variety of methods. Depending on the method used, the breeding strategy must be modified not only for the initial founding generation of mouse, but to establish individual mouse lines as well. Transgenic founder mice are each unique and must be outcrossed to initially establish the line. Mice that have been targeted using embryonic stem cells will need to be tested for germ line transmission of the modified gene prior to establishing a line. Mice that have been targeted using CRISPR/Cas9 gene-editing endonucleases will often be mosaic, and thus, also require testing for germ line transmission of the mutation of interest.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages163-169
Number of pages7
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2066
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Fingerprint

Breeding
Clustered Regularly Interspaced Short Palindromic Repeats
Germ-Line Mutation
Endonucleases
Embryonic Stem Cells
Germ Cells
Transgenic Mice
Genes

Keywords

  • Chimera
  • CRISPR
  • CRISPR/Cas9
  • Germ line transmission
  • Knock-in mice
  • Knock-out mice
  • Mosaic
  • Transgenic

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Parker-Thornburg, J. (2020). Breeding Strategies for Genetically Modified Mice. In Methods in Molecular Biology (pp. 163-169). (Methods in Molecular Biology; Vol. 2066). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9837-1_14

Breeding Strategies for Genetically Modified Mice. / Parker-Thornburg, Jan.

Methods in Molecular Biology. Humana Press Inc., 2020. p. 163-169 (Methods in Molecular Biology; Vol. 2066).

Research output: Chapter in Book/Report/Conference proceedingChapter

Parker-Thornburg, J 2020, Breeding Strategies for Genetically Modified Mice. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 2066, Humana Press Inc., pp. 163-169. https://doi.org/10.1007/978-1-4939-9837-1_14
Parker-Thornburg J. Breeding Strategies for Genetically Modified Mice. In Methods in Molecular Biology. Humana Press Inc. 2020. p. 163-169. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-9837-1_14
Parker-Thornburg, Jan. / Breeding Strategies for Genetically Modified Mice. Methods in Molecular Biology. Humana Press Inc., 2020. pp. 163-169 (Methods in Molecular Biology).
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