TY - JOUR
T1 - Bronchoalveolar lavage fluid cytology in culture-documented invasive pulmonary aspergillosis in patients with hematologic diseases
T2 - Analysis of 67 episodes
AU - Fernández-Cruz, A.
AU - Magira, E.
AU - Heo, S. T.
AU - Evans, S.
AU - Tarrand, J.
AU - Kontoyiannis, D. P.
N1 - Publisher Copyright:
© Copyright 2018 American Society for Microbiology. All Rights Reserved.
PY - 2018/10
Y1 - 2018/10
N2 - There is a paucity of studies on the yield of Gomori-methenamine-silver (GMS) staining in bronchoalveolar lavage (BAL) fluid cytology and its comparison with fluorescent dye staining for the diagnosis of invasive pulmonary aspergillosis (IPA) in patients with hematologic malignancies. To that end, we analyzed the yield of direct fungal visualization in BAL fluid cytology with GMS staining, in a series of culture-positive IPA cases in 67 patients with hematologic malignancies, and we compared the results with those of direct examination with calcofluor white staining and BAL fluid galactomannan assays, when available. GMS staining in BAL fluid cytology was positive in 42% of the 67 cases and revealed coinfections in 7 cases. In contrast, only 2/67 (3.6%) BAL fluid samples were positive in direct smears stained with the fluorescent dye calcofluor white. Positive GMS staining results were significantly more frequent in IPA cases with cavitary lesions and IPA cases caused by <1 Aspergillus species, but the proportions of positive cytology results among Aspergillus species were not different.
AB - There is a paucity of studies on the yield of Gomori-methenamine-silver (GMS) staining in bronchoalveolar lavage (BAL) fluid cytology and its comparison with fluorescent dye staining for the diagnosis of invasive pulmonary aspergillosis (IPA) in patients with hematologic malignancies. To that end, we analyzed the yield of direct fungal visualization in BAL fluid cytology with GMS staining, in a series of culture-positive IPA cases in 67 patients with hematologic malignancies, and we compared the results with those of direct examination with calcofluor white staining and BAL fluid galactomannan assays, when available. GMS staining in BAL fluid cytology was positive in 42% of the 67 cases and revealed coinfections in 7 cases. In contrast, only 2/67 (3.6%) BAL fluid samples were positive in direct smears stained with the fluorescent dye calcofluor white. Positive GMS staining results were significantly more frequent in IPA cases with cavitary lesions and IPA cases caused by <1 Aspergillus species, but the proportions of positive cytology results among Aspergillus species were not different.
KW - GMS
KW - Hematologic malignancy
KW - Invasive aspergillosis
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U2 - 10.1128/JCM.00962-18
DO - 10.1128/JCM.00962-18
M3 - Article
C2 - 30021823
AN - SCOPUS:85054194913
SN - 0095-1137
VL - 56
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 10
M1 - e00962
ER -