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Characterization of conventional dendritic cell populations in preclinical tumor models using flow cytometry

  • Erik Wennerberg
  • , Nils Petter Rudqvist
  • , Sheila Spada

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Tumor infiltration of conventional dendritic cells has been shown to be essential for triggering efficient antitumor immune responses. These findings have generated an increasing demand for reliable methods to accurately identify and quantify specific DC-subpopulations, both in immune monitoring of clinical trial samples as well as in preclinical mouse tumor models. Here, we describe a flow cytometric approach to assess percentages and absolute counts of conventional dendritic cells in solid mouse tumors.

Original languageEnglish (US)
Title of host publicationTumor Immunology and Immunotherapy - Integrated Methods Part A
EditorsLorenzo Galluzzi, Nils-Petter Rudqvist
PublisherAcademic Press Inc.
Pages139-148
Number of pages10
ISBN (Print)9780128186770
DOIs
StatePublished - 2020
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume635
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Conventional dendritic cells
  • Flow cytometry
  • Tumor microenvironment

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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